摘要
目的:探讨尼美舒利诱导人肝癌SMMC-7721细胞凋亡的作用及其机制。方法:以不同浓度的尼美舒利处理体外培养的人肝癌SMMC-7721细胞,MTT法检测细胞活力,流式细胞术检测细胞凋亡率,RT-PCR和/或Western blot法检测caspase-9和PARP的剪切情况及HSP70的表达水平;通过RNAi技术沉默HSP70基因表达,观察其对细胞凋亡的影响。结果:尼美舒利可抑制人肝癌SMMC-7721细胞生长,诱导细胞凋亡,导致caspase-9和PARP的剪切活化及HSP70的mRNA和蛋白表达下调;在人肝癌SMMC-7721细胞中转染靶向HSP70的siRNA可沉默HSP70表达、剪切活化caspase-9和PARP,进而显著促进细胞凋亡。结论:尼美舒利可诱导人肝癌SMMC7721细胞凋亡,这一作用可能与抑制HSP70表达有关。
AIM：To investigate the effect of nimesulide on cell apoptosis and possible mechanism in human hepatocellular carcinoma SMMC-7721 cells.METHODS： SMMC-7721 cells were treated with nimesulide at different concentrations.Cell viability was assessed by MTT assay.Cell apoptosis rate was determined with flow cytometry.The cleavage activity of PARP and caspase-9 and the expression of HSP70 were evaluated using RT-PCR and Western blotting.The influence of HSP70 on cell apoptosis was observed using RNA interference silencing HSP70 expression.RESULTS：Nimesulide significantly inhibited cell growth in SMMC-7721 cells in a time-and concentration-dependent manner,and induced cell apoptosis in a concentration-dependent manner.Moreover,nimesulide promoted the cleavage of caspase-9 and PARP and inhibited the mRNA and protein expression of HSP70.Through the specific inhibition on HSP70 gene with siRNA,cell apoptosis increased,and the apoptosis was enhanced by the cleavage activity of caspase-9 and PARP.CONCLUSION： Nimesulide could inhibit cell growth and induce apoptosis in human hepatocellular carcinoma SMMC-7721 cells via the downregulation of HSP70.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2012年第9期933-936,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金(81071897)
关键词
尼美舒利
肝癌
热休克蛋白70
细胞凋亡
nimesulide； hepatocellular carcinoma； heat shock protein 70； apoptosis
