摘要
目的探讨谷氨酸脱羧酶抗体(GAD-Ab)、胰岛细胞抗体(ICA)、胰岛自身抗体(IAA)和蛋白酪氨酸磷酸酶抗体(IA-2-Ab)联合检测对青壮年糖尿病患者的应用价值。方法采用免疫印迹法检测212例青壮年糖尿病患者和40例健康人血清中GAD-Ab、ICA、IAA和IA-2-Ab,并对4项自身抗体全阴患者与有1项以上阳性患者的空腹血糖、餐后2h血糖、糖化血红蛋白等血液生化指标进行比较。结果 212例青壮年糖尿病患者GAD-Ab、ICA、IAA和IA-2-Ab的阳性率分别为29.2%、21.7%、13.2%和11.3%,4项自身抗体全阴患者126例,占59.4%,与有1项以上阳性患者比较,其空腹血糖、餐后2h血糖和糖化血红蛋白水平差异无统计学意义(P>0.05)。结论4项联合检测对青壮年糖尿病患者的诊断及治疗方案的制定有重要意义。
Objective To explore value of combined detection of glutamic acid decarboxylase antibody（GAD- Ab） ,islet cell antibody（ICA）,insulin antoantibody（IAA） and IA-2 antibody in middle-age patients with diabetes mellitus（DM）. Methods 212 middle-age patients with diabetes mellitus（DM） serum GAD-Ab,ICA,IAA and IA-2- Ab were examined by Western blotting, and biochemical indicators as FPG, PBG and H bAlc were compared between antibody positive group and antibody negative group. Results In middle-age patients with diabetes mellitus（DM）, the positives rate of GAD, ICA, IAA,IA-2-Ab were 29.2 ~, 21.7 %, 13.2 %, 11.3 %. There was no differences in FPG, PBG and HbAlc of antibody positive group and antibody negative group. Conclusion The detection of GAD-Ab, ICA, IAA and IA-2-Ab antibody in serum have the important clinical significance in middle age patients with diabetes mellitus.
出处
《检验医学与临床》
CAS
2012年第18期2262-2263,共2页
Laboratory Medicine and Clinic
