摘要
目的探索肝动脉臭氧化碘油栓塞治疗新西兰大白兔VX2肝癌的安全性及疗效。方法将碘化油经臭氧化2h后备用。兔VX2肝癌模型21只．采用MRI或CT增强扫描确定肿瘤存在并测量肿瘤大小。将实验兔随机分成经肝动脉臭氧化碘油栓塞组（A组）、经肝动脉碘化油栓塞组（B组）、假手术组（C组），每组7只。治疗1周后检测血清中丙氨酸转氨酶、天冬氨酸转氨酶、白细胞和总抗氧化能力水平；治疗后每2周行CT和MRI复查，评价相对肿瘤体积，并观察组间生存期及并发症差异。结果所有动物完成相应治疗操作。处理后28d，A、B、C组相对肿瘤体积分别为1．39±1．48，7．21±5．50，12．2±7．12，三组间差异有统计学意义（X^2=10．71，P=0．005），A组和B组间差异亦有统计学意义（x^2=7．00，P=0．03）。A、B及C组平均生存时间分别为（87．14＋9．72）d、（56．14±6．81）d和（46．43±6．13）d。生存分析曲线显示A组累计生存率明显高于B组和C组。结论初步研究表明肝动脉内臭氧化碘油栓塞与单纯碘油栓塞相比可有效抑制兔VX2肿瘤生长，延长荷瘤兔生存期。
Objective To evaluate the safety and efficacy of transhepatic arterial embolization with ozonated-Lipiodol in treating VX2 carcinoma in New Zealand rabbits. Methods The Lipiodol was ozonated for 2 hours, and then stored at 4℃ temperature for future use. Twenty-one rabbits with VX2 liver carcinomas were randomly divided into three groups after the tumor was established, and the tumor＇s size was determined on MRI or enhanced CT images. Rabbits in group A （n = 7） received tran- sarterial ozonated-Lipiodol embolization. A total amount of 0.25 ml ozonated-Lipiodol was injected into the right or left hepatic tumor-feeding artery. Rabbits in group B （n = 7） received transarterial Lipiodol embolization. A total amount of 0.25 ml Lipiodol was injected into the right or left hepatic tumor-feeding artery. Group C （n = 7） was used as sham operation group. The serum levels of alanine transaminase （ALT）, glutamic-oxaloacetic transaminase （AST）, leucocyte and the total antioxidant capacity （T-AOC）were estimated one week after the treatment. Follow-up examinations with CT and MRI were performed every two weeks after the treatment to evaluate the tumor＇s volume. The survival rates and the complications of the three groups were documented and were compared between each other. Results The therapeutic procedure was successfully completed in all the animals. Twenty-eight days after the treatment, the relative tumor volume of group A, B and C was 1.39 ＋ 1.48, 7.21 ＋ 5.50 and 12.2 ＋ 7.12, respectively. The differences among the three groups were stati-stically significant （x^2= 10.71, P = 0.005）, and the difference between group A and B was also statistically significant （X^2 = 7.00, P = 0.03）. The mean survival time of group A, B and C was （87.14 ＋ 9.72） days, （56.14 ＋ 6.81） days and （46.43 ＋ 6.13） days, respectively. The Kaplan Meier survival analysis curve showed that the cumulative survival rate of group A was significant higher than those of group B and group C. Conclusion Compared with pure transartcrial Lipiodol embolization, transarterial ozonated- Lipiodol embolization can more effectively inhibit liver VX2 carcinoma growth in rabbits and elongate the tumor-bearing rabbit＇s survival time.（J Intervent Radiol, 2012, 21： 760-764）
出处
《介入放射学杂志》
CSCD
北大核心
2012年第9期760-764,共5页
Journal of Interventional Radiology
基金
上海市自然科学基金资助(10ZR1418900)
关键词
肝肿瘤
臭氧化碘油
动脉栓塞
动物实验
liver tumor
ozonated-Lipiodol
arterial embolization
animal experiment
