摘要
稻米蛋白质含量是水稻（Oryza sativa L．）营养品质育种的重要内容之一。本研究以华恢3号与中国香稻构建的重组自交系（RILs）群体为材料，构建了含有156个SSR标记的遗传连锁图谱，结合近红外分析仪测定两年（2009年，2010年）的糙米蛋白质含量，进行了QTL定位和遗传基础分析研究。两年共定位到3个控制糙米蛋白含量的QTL和16对上位性互作的位点。其中检测到的3个QTLs，分别位于第4、6、8染色体上，单个QTL解释的表型变异率为3．39％～34．2％，两年解释的总表型变异率分别为41．2％和5．95％，这些QTL中只有位于第8染色体短臂在区问RM310-RM547的qbpc8在2009年和2010年被重复检测到。LOD值分别为22．5和3．5，解释表型变异率分别为34．2％和5．95％。这些QTL的发掘，为分子标记辅助选择改良水稻营养品质奠定了基础。
The brown rice protein content （BPC） has been a major concern in rice （Oryza sativa L.） nutritional quality breeding. In this study, a linkage map consisting of 156 SSR （Simple Sequence Repeat） markers was constructed from a recombinant inbred line （RIL） population derived from cross-breeding between Zhongguoxiangdao and Huahui3. Based on the results of BPC phenotypes of RILs from both 2009 and 2010 by Near-infrared Papid Content Analyzer, more than 16 pairs of epistatic interaction loci, and a total of three quantitative trait loci （QTLs） were detected on chromosome 4, 6 and 8, respectively. Each QTL explains 3.39% to 34.2% phenotypic variation. and it explains a total of 41.2% and 5.95% phenotypic variation for 2009 and 2010, respectively. The QTL （qbpc8） flanked by RM310 and RM547 on chromosome 8 was the largest （only） one detected in both years, with LOD values of 22.5 and 3.5 andexplains 34.2% and 5.95% of phenotypic variation, respectively. These results will provide useful genetic basis for marker-assisted selection in rice quality breeding programs.
出处
《分子植物育种》
CAS
CSCD
北大核心
2012年第5期594-599,共6页
Molecular Plant Breeding
基金
国家科技部863项目(2010AA101801)
农业部水稻产业技术体系(CARS-01-03)
国家植物转基因专项(2011ZX08009-002)
国家自然科学基金(30971550)共同资助
