摘要
目的:研究北京地区1992年-2011年沙门菌菌群、血清型和耐药性的变迁,为本地区的流行病学研究及临床合理用药提供依据。方法:对腹泻病人的大便标本用SS琼脂培养,得到的疑似沙门菌用VITEK细菌鉴定仪、微量编码生化反应管和血清凝集法进行细菌鉴定和分型;采用K-B纸片法检测药物敏感性,根据CLSI标准进行判断。结果:近20年间我院共分离沙门菌709株,包含6个群、6个亚群、44个血清型,后10年有10个新的血清型出现。药物敏感性监测,20年间沙门菌对多种抗生素的耐药率有提高,但对氨苄西林、头孢噻肟、氯霉素、复方新诺明在前后两个10年间耐药率升高显著(P<0.05)。沙门菌感染年龄分布主要集中在0岁～30岁之间,流行季节集中在7月-9月。结论:北京地区1992年-2011年间沙门菌血清型种类有变化,部分抗生素的耐药率增加明显,应加强监测,正确防治。
Objective：To study the changes of serotype distribution and antibiotic sensitivity of Salmonella in Beijing from 1992 to 2011,and provide basis for epidemiology research and rational drug use in clinic.Methods： SS agar was used to culture stool specimens from patients with diarrhea.VITEK,trace-coding biochemical reaction tube and serum aggregation method were used for bacterium identification.Drug resistance test was then performed by K-B method and the conclusion was drawn according to CLSI.Results： 709 Salmonella strains including 6 groups,6 subgroups and 44 serotypes were isolated during recent 20 years.The resistant rates to most antibiotics were increasing and resistant rates to Ampicillin,Cefotaxime,chloromycetin and Trimethoprim-sulfamethoxazole increased obviously during 20 years（P0.05）.Salmonella infection mainly concentrated on population between 0-30 years old during July to September.Conclusion： Salmonella serotypes have taken changes and the resistant rates to some antibiotics increased significantly during 20 years in Beijing.It is necessary to strengthen supervision for prevention and treatment of Salmonella infection.
出处
《中国卫生检验杂志》
北大核心
2012年第9期2217-2219,共3页
Chinese Journal of Health Laboratory Technology
关键词
沙门菌
血清型
耐药
Salmonella
Serotype
Resistance
