摘要
本研究以α—^(32)P—dCTP标记的c—myc,c—fos和c—Ha—ras癌基因为探针,应用限制性内切酶技术和Southern印迹杂交方法对15例人乳头瘤病毒16型(HPV—16)基因组已发生整合的宫颈癌标本中上述三种癌基因进行了分析。结果表明,在含有HPV—16基因组整合的大多数宫颈癌组织(10/15)中,细胞myc(c—myc)原癌基因表现出不同程度的扩增或(和)重排,个别宫颈癌组织同时存在着c—ras原癌基因的重排,而HPV—16阳性但未发生整合的2例正常宫颈组织中(亚临床乳头瘤病毒感染)无类似的分子改变,提示c—myc原癌基因的扩增或(和)重排可能与HPV—16的整合有关,并协同参与致癌过程。
The Physical state of 32 cases of cervical proliferative lesions which were positive to dot blot hybridization of HPV—16DNA were further analyzed by using labelled HPV—16 DNA probe, restriction enzyme technique and Southern blot hybridization method. The results showed that HPV—16 DNA sequences could only be detected as an episome in normal cervical epithelia and cervical benign proliferative lesions(cervical condyloma),on the contray, HPV—16 DNA was found to have been integrated into the genome of host cell in of cervical malignant proliferative lesions(cervical cancer) while in the cervical malignant lesions(cervical intraepithelial neoplasia, CIN), HPV—16 DNA sequences coexisted in form of episome and integration. These results suggest that integration of HPV—16 genome in cervical epithelia may play an important role in the process of occurrence and development of cervical cancer.
出处
《遵义医学院学报》
1990年第2期3-7,共5页
Journal of Zunyi Medical University
关键词
宫颈癌
人乳头瘤病毒
癌基因
cervical cancer
cervical condyloma
cervical intraepithelial neoplasia
integrating state
DNA probe
Southern blot hybridization
