摘要
目的探讨基质金属蛋白酶-25（MMP-25）在胃癌中的表达及临床意义。方法采用免疫组织化学技术和逆转录一聚合酶链反应（RT—PCR）检测40例正常胃组织、40例萎缩性胃炎组织、40例胃癌组织中MMP-25的表达。结果免疫组织化学中，MMP-25在胃癌组织和萎缩性胃炎组织中的阳性率均明显高于正常胃组织（95．0％、82．5％、22．5％，P〈0．05）；RT—PCR中，MMP-25在胃癌组织和萎缩性胃炎组织中的表达率均明显高于正常胃组织（0．328±0．005、0．175±0．002、0．103±0．004，P〈0．05）。MMP-25在癌组织中表达与肿瘤浆膜浸润、淋巴结转移及TNM分期明显相关（P〈0．05），与患者年龄、性别、肿瘤大小、分化程度等无明显相关（P〉0．05）。结论MMP-25在胃癌组织中高表达，与肿瘤的发生、转移有密切关系。
Objective To investigate the expression and clinical significance of matrix metallpro- teinase-25 （MMP-25） in gastric carcinoma. Methods The expression of MMP-25 in 40 cases of normal gastric tissue, 40 cases of atrophic gastritis and and 40 cases of gastric carcinoma was detected by using immunohistochemistry and reverse transcription-polymerase chain reaction （RT-PCR） separately. Results The expression rate of MMP-25 protein in gastric carcinoma and atrophic gastritis was significantly higher than in the normal gastric tissues （95.0% and 82. 5% vs. 22. 5%, P 〈 0. 05 ）, and that of MMP-25 mRNA showed the same trend as the former （0. 328 ±0. 005 and 0. 175 ±0. 002 vs. 0. 103±0. 004,P 〈 0. 05 ）. The expression of MMP-25 was significantly Correlated with the clinicopathologieal factors such as depth of invasion, lymph node metastasis and tumor sizes （P 〈 0. 05 ）, but not with age, gender, clinical stage and differentiation （P 〉 0. 05 ）. Conclusion The MMP-25 is expressed distinctly in gastric carcinoma. MMP-25 may be involved in tumorigenesis and metastasis of gastric carcinoma, and expected to be an important biomarker for gastric carcinoma, which could provide clues for clinical diagnosis of gastric carcinoma.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第10期1997-1999,I0002,共4页
Chinese Journal of Experimental Surgery
关键词
基质金属蛋白酶-25
胃癌
转移
Matrix metallproteinase-25
Gastric carcinoma
Metastasis
