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体外原代培养脐带基质间充质细胞和人皮肤成纤维细胞 被引量：2

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摘要 目的探讨体外原代培养脐带基质间充质细胞(UMC)和人皮肤成纤维细胞(HSF)的方法。方法用Ⅳ型胶原蛋白酶、Ⅰ型脱氧核糖核酸酶和0.25%胰蛋白酶消化法原代分离培养UMC细胞,用组织块贴壁法原代分离培养HSF细胞,镜下观察细胞的培养过程和生长状态。结果脐带组织分离培养第3天,有少量UMC贴壁生长,细胞膜周围有折光性,形态类似于成纤维细胞;皮肤组织块贴壁培养第7天,有HSF爬出,呈长梭形、不规则三角形。随着细胞培养时间延长,UMC和HSF数量逐渐增多,细胞生长状态良好。结论应用酶消化法和组织块贴壁法可成功分离培养出UMC和HSF。
作者 蒋知新 艾民 张清华 沙杭 高毅 卢海
机构地区 中国人民解放军三○五医院 南方医科大学 南方医科大学再生医学研究所
出处 《中国老年学杂志》 CAS CSCD 北大核心 2012年第19期4193-4194,共2页 Chinese Journal of Gerontology
基金 全军医药卫生科研基金项目(No.08Z017) 科学技术部867计划项目(No.2006AA02A141)
关键词 细胞培养 脐带基质间充质细胞 人皮肤成纤维细胞
分类号 R39 [医药卫生—基础医学]
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参考文献5

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同被引文献29

  • 1卢润章,赵经慧,程梅,王志国,王洪岩,陈立君,马军.人脐带间充质干细胞体外培养的生物学特性研究[J].黑龙江医学,2013,37(2):81-83. 被引量：4
  • 2Rohaina CM, Then KY, Ng AM, et al. Reconstruction of limbal stem cell deficient corneal surface with induced human bone marrow mesenchymal stem cells on amniotic membrane. Transl Res. 2014; 163(3):200-210.
  • 3Niess H, Bao Q, Conrad C, et al. Selective targeting of genetically engineered mesenchymal stem cells to tumor stroma microenvironments using tissue-specific suicide gene expression suppresses growth of hepatocellular carcinoma. Ann Surg. 2011;254(5): 767-774; discussion 774-775.
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  • 5Sun JH, Zhang YL, Qian SP, et al. Assessment of biological characteristics of mesenchymal stem cells labeled with superparamagnetic iron oxide particles in vitro. Mol Med Rep. 2012;5(2):317-320.
  • 6Shaer A, Azarpira N, Aghdaie MH, et al. Isolation and characterization of Human Mesenchymal Stromal Cells Derived from Placental Decidua Basalis; Umbilical cord Wharton's Jelly and Amniotic Membrane. Pak J Med Sci. 2014;30(5):1022-1026.
  • 7Balasubramanian S, Thej C, Venugopal P, et al. Higher propensity of Wharton's jelly derived mesenchymal stromal cells towards neuronal lineage in comparison to those derived from adipose and bone marrow. Cell Biol Int. 2013;37(5):507-515.
  • 8Ribeiro J, Pereira T, Amorim I, et al. Cell therapy with human MSCs isolated from the umbilical cord Wharton jelly associated to a PVA membrane in the treatment of chronic skin wounds. Int J Med Sci. 2014;11(10): 979-987.
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引证文献2

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中国老年学杂志
2012年 第19期

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