摘要
通过毛细辛不同溶剂提取物体外抗氧化活性研究,为进一步开发毛细辛的药用价值提供参考。用50%的乙醇回流提取毛细辛的粉末,减压浓缩过滤得到乙醇浸膏,加蒸馏水制成悬浮液,依次用等体积的石油醚,乙酸乙酯,正丁醇萃取,得到石油醚,乙酸乙酯,正丁醇和水提物。以2,6-二叔丁基对甲酚(BHT)为对照,分别采用DPPH法、Fenton法、NBT还原法和Fe2+螯合法测定4种提取物的抗氧化活性。结果表明,毛细辛4种溶剂提取物均有不同程度的抗氧化活性,且活性与浓度成正相关。毛细辛乙酸乙酯提取物的1,2-二苯基-2-苦苯肼自由基(DPPH.)的清除活性(IC50值0.376mg/mL),超氧阴离子自由基(O2-.)的清除活性(IC50值0.297mg/mL)均最强。正丁醇提取物的羟基自由基(.OH)清除活性(IC50值0.776mg/mL)为最强,水提物的金属离子螯合活性(IC50值0.026mg/mL)为最强,远超过对照BHT(0.373mg/mL)。实验表明,毛细辛乙酸乙酯提取物,正丁醇提取物和水提物均有较强的自由基清除活性,可作为新的自由基清除剂;水提物可以作为新的金属离子螯合剂。
The antioxidative activities in vitro of different solvent extracts of Asarum himalaicum were studied to provide more information for future research on the medicinal value of A.himalaicum.The powdered material of A.himalaicum was extracted with 50% ethanol refluxing extraction and the ethanol condensate was evaporated to dryness under vacuum on a rotary evaporator.The condensate was then extracted with Petroleum ether,ethyl acetate or n-butanol or water at the same volume to get four different extracts.The four extracts were then examined for their antioxidative activities by DPPH（1,1-diphenyl-2-plcrylhydrazyl）,Fenton,NBT（nitrotetrazolium blue chloride） reduction and Fe2＋ chelation.The results suggest that the four solvent extracts of A.himalaicum had a certain degree of antioxidant activity,and the activity had a positive correlation with the concentration of the extract.The ethyl acetate extract had higher activity in scavenging DPPH·（IC50 =0.376 mg/mL） and O2-·（IC50=0.297 mg/mL Hile the n-butanol extract had the highest ·OH scavenging capacity（IC50=0.776 mg/mL）.The water extract was the most powerful for chelating metal ions（IC50=0.026 mg/mL） and was more powerfull than that of BHT（2,6-di-tert-butyl-4-methylp-henol）（IC50=0.373 mg/mL）.This study has shown that the ethyl acetate extract,n-butanol extract and water extract of A.himalaicum have remarkable free radical scavenging activities,and the water extract can be selected as a new metal ion chelating reagent.
出处
《草业学报》
CSCD
北大核心
2012年第5期285-290,共6页
Acta Prataculturae Sinica
基金
国家自然科学基金项目(30771454
31172365
31170366)资助
关键词
毛细辛
提取物
抗氧化活性
自由基清除
Asarum himalaicum
extract
antioxidant activity
free radical scavenging
