摘要
目的研究促红细胞生成素(EPO)对大鼠缺血再灌注后核因子-κB(NF-κB)表达的影响。方法将45只SD大鼠随机分为假手术组、脑缺血再灌注模型组(模型组)以及EPO治疗组(治疗组)。构建SD大鼠大脑中动脉闭塞脑缺血/再灌注模型,观测缺血再灌注后4、12、24h的神经功能缺损评分;比较脑梗死体积,免疫组织化学检测NF-κB蛋白的表达,TUNEL法检测神经元凋亡率。结果与模型组比较,治疗组12、24h的神经功能缺损评分降低;脑梗死体积显著减小;脑内NF-κB蛋白的表达与神经元凋亡率明显降低。结论 EPO可通过降低NF-κB蛋白表达对大鼠脑缺血再灌注损伤起保护作用。
Objective To observe the effect of erythropoietin on the expression of nuclear factor-κB in brain of rat during ischemia-reperfusion injury.Methods 45 rats were randomly divided into sham operation group,model group and therapeutic group.Model mice of middle cerebral artery occlusion with ischemia-reperfusion injury was established.The score of the deficient neural function and the behavior were observed at 4 h,24 h and 72 h hour after ischemia-reperfusion;the volume of cerebral infraction was compared;the expression of NF-κB and neurona poptosis in brain tissue were detectived by Immunohistoehemistry and TUNEL.Results The volumes of cerebral infarcts,expression of NF-κB and The proportion of apoptosis in therapeutic groups were lower than that in model group;the neural function grades and the behavior grades therapeutic group were lower than that in model group in 12 h and 24 h.Conclusion EPO might protect neurons by markedly reduce the expression of NF-κB of the rats with ischemia-reperfusion.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第30期3192-3193,3197,共3页
Chongqing medicine
