摘要
目的通过对367例HIV-1抗体阳性者的蛋白印迹法(WB)的抗体条带分析,了解不同HIV-1感染人群其抗体WB带型分布的差异。方法采用WB法对HIV初筛有反应性样本进行HIV-1抗体确认,WB阳性者按照不同年龄、性别、民族三组进行WB带型的统计分析。结果 HIV-1gag基因编码的P39和P17抗体的阳性率在女性中明显高于男性(χ2值分别为6.386、10.872,均P<0.05);年龄小于或等于50岁的HIV-1抗体阳性者gag基因编码的P39和pol基因编码的P31抗体阳性率分别明显高于年龄超过50岁者(χ2值分别为6.882、4.360,均P<0.05);而HIV-1抗体WB带型分布在不同民族之间的差异无统计学意义。结论 HIV-1抗体WB带型分布在不同性别、不同年龄及不同民族人群间差异无统计学意义,HIV-1pol及gag基因编码的P39和P17蛋白的抗体产生在不同性别、不同年龄的人群间差异有统计学意义。这对HIV感染后病原检测和诊断包括传染源确定、传播途径追踪以及临床鉴别诊断具有非常重要的意义。
Objective To understand the distribution difference of Western blot（WB） banding patterns in dif- ferent HIV-1 infected population by analyzing the WB banding patterns in 367 cases of HIV-1 antibody positive. Methods The samples showing reaction in primary screening of HIV were verified by WB. The WB positive individ- uals were divfded into 3 groups according to different ages, sexes and nationalities for performing the WB banding patterns analysis. Results The positive rate of P39 and P17 anti-body in HIV-1 gag genetic encoding was obviously higher in females than in males（χ2=6. 386,10. 872,P〈0.05）. The positive rate of P39 anti-body in HIV-1 gag ge- netic encoding and P31 anti-body in pol genetic encoding in HIV 1 positive individuals aged ≤ 50 years was signifi- cantly higher than that in HIV-1 positive individuals aged〉50 years（χ2=6. 882,4. 360 ,P（0.05）. The WB banding pattern distribution of HIV-1 antibody had no statislical difference among different nationalities. Conclusion The Wig banding pattern distribution of HIV-1 antibody has no statistical difference among different sexes,different ages and different nationalities,and the antibody production of P39 and P17 in HIV-1 pol or gag genetic encoding has sta- tistical difference among different sexes and different ages, which is very important significant to the pathogen detec- tion after HIV infection and diagnosis including determining the source of infection, tracking the transmission route and clinical difterential diagnosis.
出处
《检验医学与临床》
CAS
2012年第20期2529-2530,共2页
Laboratory Medicine and Clinic
基金
"十一五"国家重大科技专项"艾滋病和病毒性肝炎等重大传染病防治"资助课题资助项目(2008ZX10001-008)
