摘要
目的了解十堰市健康人群口咽部流感嗜血杆菌(Hi)携带情况,为有效防控Hi感染提供依据。方法随机抽取十堰市5个年龄组202份健康人群的咽拭子标本,Hi携带检测采用荧光定量PCR法。结果 202份咽拭标本Hi阳性110份,阳性率为54.46%。其中3岁～年龄组阳性率最高,为76.92%(50/65),占总阳性数的45.45%(50/110);≥20岁人群阳性率最低,为15.62%;各年龄组人群Hi阳性率差异有统计学意义(χ2=40.03,P<0.01);男、女咽拭子标本PCR阳性率分别为52.78%(57/108)和56.38%(53/94);b型流感嗜血杆菌(Hib)阳性率为8.42%(17/202),其他型别和不可分型流感嗜血杆菌阳性率为46.04%(93/202)。结论十堰市健康人群Hi携带率较高;在重视Hib疫苗接种的同时,应加强Hi新型疫苗的研究。
Objectives To ascertain the prevalence of carriers of Haemophilus influenza（Hi） in the mouth and throat among the healthy population in the city of Shiyan and to provide evidence for effective prevention and control of Hi infection.Methods Throat swab specimens were randomly collected from 202 healthy individuals from five age groups in the city of Shiyan.The prevalence of carriers of Hi was monitored using TaqMan quantitative PCR.Results Of the 202 specimens,110 tested positive at a rate of 54.46%.The specimens that tested positive most often（76.92%,50/65 specimens）were from children ages 3 and up.These children accounted for 45.45%（50/110）of all positive specimens.The specimens that tested positive least often（15.62%） were from young adults age 20 and over 20 years.There are the significant differences（χ2=40.03,P0.01） in the positive rate for different age groups.Of the throat swab specimens that tested positive according to PCR,52.78%（57/108） were from males while 56.38%（53/94） were from females.Positivity for Haemophilus influenza type B（Hib） was 8.42% while positivity for other types and unknown types of Hi was 46.04%.Conclusion The healthy population in the city of Shiyan tested positive for Hi at a relatively high rate.Hib vaccines should be emphasized and research on new vaccines should be enhanced.
出处
《中国病原生物学杂志》
CSCD
北大核心
2012年第10期784-786,共3页
Journal of Pathogen Biology
关键词
健康人群
流感嗜血杆菌
荧光定量PCR
Healthy population
Haemophilus influenzae
taqman quantitative PCR
