摘要
目的:研究异补骨脂素(Isopsoralen,ISR)和蜕皮甾酮(Ecdysterone,ECR)对经H2O2处理的HLEC内磷酸化ERK(p-ERK)的表达,从而探讨雌二醇(β-Estradiol,E2),ECR和ISR是否通过启动或干预ERK/MAPK信号转导途径来发挥抗氧化损伤作用,以揭示E2和ECR及ISR抗氧化损伤的信号转导机制。方法:本研究采用ECR和ISR与人晶状体上皮细胞(HLEC)共同孵育,以E2作为阳性对照,再用H2O2对HLEC造成氧化损伤后,采用流式细胞术(flow cytometer,FCM)检测不同时段的p-ERK蛋白表达。结果:正常HLEC内存在p-ERK表达;H2O2组p-ERK表达量随时间的延长而减少;E2和ECR与ISR各组p-ERK表达量分别在1,6,12h达到高峰。结论:E2和ECR与ISR的抗氧化损伤作用可能是与ERK/MAPK信号通路有关。
AIM：To investigate the relative mechanisms of signal transduction of isopsoralen（ISR） and ecdysterone（ECR） on phosphorylated extracellular signal-regulated kinase（p-ERK） expression in human lens epithelial cells（HLEC）,which will provide a view in better understanding the mechanisms of aged cataract,and provide the experiment bases for the prophylaxis and treatment of aged cataract.METHODS：Human lens epithelial cells（HLECs） were cultured and sub-cultured in vitro.The cultured HLECs were exposed to H2O2 at 300μmol/L over a time course of several hours,with and without pretreatment with β-Estradiol（E2）,ECR,ISR.The HLECs were used for following experiments.The changes of the expression levels of ERK phosphorylation in HLECs which exposed to H2O2,with pretreatment with certain concentration of ECR and ISR,were analyzed by flow cytometer（FCM）,the signal transduction mechanism of ECR and ISR on antioxidative damage was studied.RESULTS：：The expression levels of ERK phospho-rylation were examined in normal HLECs by FCM,and peaked at 6 hour.The p-ERK levels of H2O2 group gradually decreased with a prolongation of treatment time.There were significant differences as compared with control group respectively（P0.01）.The p-ERK levels of E2 group gradually increased with a prolongation of treatment time,showed significant increase from 6 hour when compared with H2O2 group respectively（P0.01）,and peaked at 6 hour.The p-ERK levels of ECR group showed significant increase from 3 hour when compared with H2O2 group respectively（P0.01）,and peaked at 12 hour.The p-ERK levels of ISR group showed significant increase from 1 hour when compared with H2O2 group respectively（P0.01）,and peaked at 1 hour.CONCLUSION：The resistance against oxidant-induced injury by E2,ECR and ISR in HLECs might relate to ERK/MAPK signaling pathway.
出处
《国际眼科杂志》
CAS
2012年第11期2072-2074,共3页
International Eye Science
