摘要
目的 :通过再狭窄动物模型的制作研究实验犬食管支架术后不同时间局部组织的形态学变化。方法 :以 16只成年健康犬为实验对象 ,均分 4组。采取“自体阔筋膜移植固定法”置入“Z”形自扩张金属食管支架 ,术后 1、2、4、8周分批处死动物 ,取出置架部位的食管组织 ,进行大体形态、光镜、电镜等病理分析。结果 :术后 1周 ,置架部位食管粘膜明显充血水肿 ,局部大量炎性细胞浸润 ,某些部位组织开始向管腔内生长 ;术后 2周 ,食管组织增生显著 ,大部分支架结构被增生组织所覆盖 ,局部有广泛肉芽组织形成及部分纤维化 ;术后 4、8周 ,增生组织已完全覆盖支架结构 ,并连接成片 ,管腔明显狭窄 ,局部出现大量的纤维结缔组织 ,炎性细胞及新生毛细血管显著减少。电镜发现 ,术后 2周组织中成纤维细胞处于旺盛的增殖及分泌状态 ,术后 8周可见大量纤维组织形成。结论 :再狭窄主要表现为肉芽组织形成及纤维化 ,炎性细胞的浸润是再狭窄形成的起始因素 ,术后 4、8周随着炎性反应的减弱 ,纤维化过程渐趋稳定。
Purpose To study the morphologic changes of esophageal tissues after stenting by making animal model of re stenosis. Methods Esophageal stent was placed by the method of “autogenous broad fascia transplantation and fixation”,the dogs were killed at the end of 1、2、4、8 weeks,and the esophageal tissue with stent were taken out and analysed by gross observation,light microscopy,electron microscopy. Results The esophageal mucosa was in the state of congestion and dropsy at week 1 after stenting,and esophageal tissue had begun to grow towards lumen in some stenting places with a great number of inflammatory cells infiltrating. Two weeks after stenting esophageal tissue proliferated greatly with much of granulation formed,most network of stent was covered by hyperplastic tissue, The proliferated tissue covered the stent completely 4、8 weeks after stenting,and connected as a body ,a great deal of fibrotic tissue had appeared,inflammatory cells and newborn capillary reduced greatly,The esophageal lumen was evidently narrow. Electron microscopy revealed that the fibroblasts was in the state of vigorous proliferation and secretion in esophageal tissue at week 2. Conclusion Re stenosis mainly expresses as granulation formation and fibrosis.Inflammatory cells infiltration is the initial factor for restenosis. At 4、8 week with the weakening of inflammatory reaction fibrosis become stable gradually.
出处
《临床与实验病理学杂志》
CAS
CSCD
2000年第1期48-51,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金资助项目!(No39670341)
