摘要
采用水培方法,研究了外源一氧化氮(NO)供体硝普钠(SNP)对300μmol.L-1LaCl3胁迫下黑麦草幼苗生长和根系活性氧代谢的影响。结果表明:在LaCl3胁迫下,喷施50μmol.L-1SNP能够抑制镧(La)从黑麦草根系向地上部的转运,缓解La对幼苗生长的抑制作用;提高幼苗根系超氧化物歧化酶和抗坏血酸过氧化物酶活性,降低过氧化物酶活性及谷胱甘肽、脯氨酸、H2O2、丙二醛含量、超氧阴离子产生速率和质膜相对透性,对过氧化氢酶活性和抗坏血酸含量无显著影响。表明NO可通过活性氧代谢的调节,缓解高浓度La胁迫对黑麦草幼苗生长的抑制作用。
Hydroponic experiments were conducted to study the effects of exogenous nitric oxide （NO） donor so- dium nitroprusside （SNP） on the growth and root reactive oxygen species metabolism in ryegrass （Lolium perenne L. ） seedlings under 300 μmol ·L^-1 LaCl3 stress. The results showed that under the stress, spraying 50 μmol · L^-1 SNP significantly inhibited the transportation of lanthanum （La） from root to shoot in ryegrass seedlings, allevia- ted the inhibition effect of La on the seedlings growth, increased the activities of superoxide dismutase and ascor- bate peroxidase, significantly decreased peroxidase activity, the contents of reduced glutathione, proline, H2O2 and malondialdehyde, and O2- production rate as well as plasma membrane relative permeability in the seedling roots, but had no effect on catalase activity and ascorbic acid content. The results indicated that NO could allevi- ate the growth inhibition of ryegrass seedlings caused by high concentration La stress via adjusting the root reac- tive oxygen species metabolism.
出处
《植物研究》
CAS
CSCD
北大核心
2012年第6期680-684,688,共6页
Bulletin of Botanical Research
基金
甘肃省庆阳市科技支撑计划项目(GK098-1-34)
关键词
一氧化氮
镧
幼苗生长
活性氧
黑麦草
nitric oxide
lanthanum
seedling growth
reactive oxygen species
ryegrass
