摘要
目的定量分析下肢动脉核因子（NF）-κB和血小板源性生长因子（PDGF）的表达，探讨其在下肢动脉硬化闭塞症患者血管壁的表达及相互关系。方法对病例组19例终末期动脉硬化的下肢动脉和对照组3例正常下肢动脉NF-κB和PDGF的表达进行实时定量聚合酶链反应（RT-qPCR）分析。结果病例组患者动脉壁NF-κB（35．42±5．69）和PDGF-A（34．38±5．80）、PDGF-B（33．95±5．92）mRNA的相对表达量均高于对照组（P〈0．05），且NF-κB与PDGF-A（r=0．926）和NF-κB与PDGF-B（r=0．925）之间均存在线性正相关（P〈0．05）。结论下肢动脉硬化闭塞症患者血管壁存在NF-κB和PDGF高表达，并且两者表达相关。
Objective To analyze the expression of nuclear faetor-KB （NF-κB） and platelet derived growth factor （PDGF） on the intima and media by using quantitative analysis, and investigate the changes in the expression patterns of NF-κB and PDGF on the vessel wall of patients with lower extremity arterial occlusive disease. Methods Nineteen patients with lower extremity arterial occlusive disease served as study group, and 3 patients with other diseases as control group, respectively. The expression of NF-KB and PDGF on the intima and media of the resected arteries was detected by using real-time polymerase chain reaction （RT-qPCR）. Results The mRNA expressions of NF-KB （35.42 ±5.69 ）, PDGF-A （34. 38±5.80） and PDGF-B （33.95 ±5.92） on the vessel wall of study group was significantly higher than that in control group （ P 〈 0. 05 ）. There was a positive linear correlation between NF-κB and PDGF-A （r=0.926） or between NF-κB and PDGF-B （r=0.925,P〈0. 05）. Conclusion There was higher expression of NF-KB and PDGF on the atheroselerotic vesssel walls of patients with lower extremity arterial occlusive disease, and a positive linear correlation between NF-KB and PDGF.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第11期2153-2155,共3页
Chinese Journal of Experimental Surgery
基金
吴阶平医学基金会临床科研资助项目（320675007123）
关键词
下肢动脉硬化闭塞症
核因子-ΚB
血小板源性生长因子
Lower extremity arterial occlusive disease
Nuclear factor-κB
Platelet-derivedgrowth factor
