摘要
对手参组织培养和植株再生的技术条件和参数进行了研究。结果表明,接近成熟的4月胚龄的种胚的萌发和成苗最具优势。PT培养基为手参胚萌发的最适培养基,在其中加入10%的马铃薯提取物可提高萌发率和成苗率,加1%的活性炭可对试管苗的生长起到促进作用。在MS培养基中加入0.4 mg/L的6-BA和0.2 mg/L的NAA,试管苗茎尖的增殖效果最佳。生根培养时在MS培养基中加0.5 mg/L NAA和0.6 mg/L IBA的效果最好,加1%的活性炭有利于生根壮苗。
The tissue culture and plant regeneration of Gymnadenia conopsea were studied.The results indicated that the 4 month embyro culture had the highest sprouting and plantlets capability.The optimal medium for the embryo culture was PT.Adding 10 % patato extract could promote embyros sprouting and plantlets formation,1 % active carbon could improve plantlets growing.MS ＋ 6-BA 0.4 mg/L ＋ NAA 0.2 mg/L had the best effectiveness for the stem tip proliferation.The best medium of rooting was MS ＋ NAA 0.5 mg/L ＋ 0.6 mg/L IBA,and 1 % active carbon could improve rooting.
出处
《西南农业学报》
CSCD
北大核心
2012年第5期1841-1844,共4页
Southwest China Journal of Agricultural Sciences
基金
四川省科技厅应用基础研究项目(2010JY0039)
关键词
手参
胚
组织培养
生根
Gymnadenia conopsea
Embryo
Tissue culture
Rooting
