摘要
目的建立同时测定复方土荆皮酊(土荆皮等)中土荆皮乙酸-O-β-D-葡萄糖苷、土荆皮丙酸、土荆皮乙酸和土荆皮甲酸4种二萜类成分的HPLC法。方法采用Agilent Zorbax SB C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈(A)-0.09%甲酸(B)为梯度洗脱流动相,体积流量1.0 mL/min,柱温35℃,检测波长为262 nm。结果土荆皮乙酸-O-β-D-葡萄糖苷、土荆皮丙酸、土荆皮乙酸和土荆皮甲酸分别在11.598～82.845μg/mL,1.206～27.009μg/mL,14.746～126.392μg/mL和0.159～2.274μg/mL范围内线性关系良好;4种成分的平均加样回收率(n=6)分别为99.0%(RSD为0.29%)、99.5%(RSD为0.15%)、101.5%(RSD为0.17%)和99.1%(RSD为0.26%)。结论该方法操作简便,准确度高,能够更好的用于复方土荆皮酊的质量控制。
AIM To establish an HPLC method for simultaneously determining the contents of pseudolaric acid B-O-β-D-glucoside,pseudolaric acid C,pseudolaric acid B and pseudolaric acid A in Compound Tujingpi Tincture（Pseudolaricis Cortex,etc.）.METHODS Agilent Zorbax SB C18 column（4.6 mm×250 mm,5 μm） was adopted.Acetonitrile（A）-0.09% formic acid（B） was used as gradient mobile phase at 35 ℃.The flow rate was 1.0 mL/min,and the detection wavelength was set at 262 nm.RESULTS The calibration curves of pseudo-laric acid B-O-β-D-glucoside,pseudolaric acid C,pseudolaric acid B and pseudolaric acid A had good linearity in the ranges of 11.598-82.845 μg/mL,1.206-27.009 μg/mL,14.746-126.392 μg/mL and 0.159-2.274 μg/mL,respectively.The average recoveries（n=6） of four diterpenoids were 99.0%（RSD of 0.29%）,99.5%（RSD of 0.15%）,101.5%（RSD of 0.17%）,99.1%（RSD of 0.26%）,respectively.CONCLUSION The developed method is convenient for the quality control of Compound Tujingpi Tincture with high accuracy.
出处
《中成药》
CAS
CSCD
北大核心
2012年第11期2124-2128,共5页
Chinese Traditional Patent Medicine
基金
军队十二五规划项目(2011-2013)
