摘要
目的探讨云南省妇女宫颈人乳头瘤病毒(HPV)感染状况、亚型及年龄分布,为该地区预防HPV感染和防治宫颈癌提供科学依据。方法收集2010年7月至2011年12月云南省各地市州县送检至昆明医学院第一附属医院及于该院妇产科门诊就诊并自愿接受宫颈HPV检查者宫颈标本3000例为研究对象,年龄为18～75岁。通过提取HPV DNA后,通过巢式PCR,美国国立生物技术信息中心(NCBI)BLAST序列比对等方法分析HPV-DNA亚型、感染率和年龄分布(本研究遵循的程序符合中国医学科学院北京和医学院医学生物学研究所人体试验委员会制定的伦理学标准,得到该委员会批准)。结果自3000例受试对象中,共计检出646例HPV呈阳性者,感染率为21.53%。其中高危型HPV(HR-HPV)为476例(73.68%,476/646),以HPV-16(33.59%,217/646),-58(22.45%,145/646),-33(8.51%,55/646)为主;低危型HPV(LR-HPV)为170例(26.32%,170/646),以HPV-6(12.54%,81/646),-81(7.74%,50/646),-11(4.43%,28/646)为主。HPV感染的高峰年龄为29～39岁(44.27%,286/646)。结论 HPV基因亚型分布具有一定地域性,云南省妇女宫颈HPV感染最常见类型为HPV-16,-58,-6。HPV-DNA分型检测在宫颈癌的临床诊断和预后方面具有重要意义。
Objective To investigate the infection rate,geno types and age distribution of human papillomavirus（HPV） in Yunnan Province.Methods From July 2010 to July 2011,a total of 3000 cervical spe cimens of outpatients from First Affiliated Hospital of Kunming Medical Universi ty were included into this study.After DNA extraction and amplification,geneti c subtypes were analyzed by nested PCR and NCBI BLAST.The age distribution was also analyzed.Informed consent was obtained form all participates.Re sults A total of 646 cases out of 3000 were HPV-positive,with the pos itive rate of 21.53%.Among 646 HPV-positive samples,high risk HPV（HR-HPV） w ere 476 cases（73.68%,476/646）,and the top three subtypes were HPV-16（33.59%,217/646）,-58（22.45%,145/646）,-33（8.51%,55/646）;low risk HPV（LR-HPV） were 170 cases（26.32%,170/646）,and the top three subtypes were HPV-6（12.54 %,81/646）,-81（7.74%,50/646）,-11（4.43%,28/646）.The peak ages of HPV inf ection was 29-to 39-year old.Conclusions The most common HP V su btypes in Yunnan Province among female reproductive tract infection were HPV-16,-58,-6.The detection of HPV-DNA is of great importance in diagnosis and prognosis of cervical cancer.
出处
《中华妇幼临床医学杂志（电子版）》
CAS
2012年第5期467-470,共4页
Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)
基金
国家自然科学基金面上项目(81171946)
云南省自然科学基金项目(2009ZC187M)
云南省科技厅社会发展科技计划项目(2011CA016)~~
