摘要
[ Objective] This study aimed to establish in vitro regeneration system for Piper methysticum. [ Method] With tender kava leaves and stems with bud as explants, different hormone combinations were added into basic medium, to screen the optimal regeneration medium. [ Result] The optimal induction medium for multiple shoots of kava was MS ＋ 4 mg/L 6-BA ＋ 0.5 mg/L GA3 ＋ 0.65% agar ＋ 3% sucrose ＋ 0.012 5% PVP; the optimal medium for elongation and rooting of shoots was 1/2MS ＋ 5 nmal/L JA ＋ 0.65% agar ＋ 3 % sucrose. [ Conclusion] This study provides theoretical guidance for the enlargement of industrial production of kava.
[ Objective] This study aimed to establish in vitro regeneration system for Piper methysticum. [ Method] With tender kava leaves and stems with bud as explants, different hormone combinations were added into basic medium, to screen the optimal regeneration medium. [ Result] The optimal induction medium for multiple shoots of kava was MS ＋ 4 mg/L 6-BA ＋ 0.5 mg/L GA3 ＋ 0.65% agar ＋ 3% sucrose ＋ 0.012 5% PVP; the optimal medium for elongation and rooting of shoots was 1/2MS ＋ 5 nmal/L JA ＋ 0.65% agar ＋ 3 % sucrose. [ Conclusion] This study provides theoretical guidance for the enlargement of industrial production of kava.
