摘要
应用巢式 -PCR方法对 3例HIV - 1感染者外周血单个核细胞中的HIV - 1膜蛋白基因进行扩增 ,并对C2 -V3及其邻区 36 0个核苷酸序列进行测定和分析 ,计算基因离散率和系统进化树。结果 ,3例感染者体内有B和C或B′和E两种不同HIV - 1亚型毒株序列 ,各亚型毒株与相应的Bcon、Ccon、Econ国际亚型毒株序的基因离散率分别为 5 0 7～ 7 2 6 %、3 2 3%、5 38～ 5 99% ;同一个体内不同亚型毒株序列间的基因离散率分别为 2 3 5 2 %、2 0 6 9%、2 8 44 % ;根据gp12 0V3环顶端四肽序列特征 ,发现有卖淫和静脉吸毒史妓女体内的sz46- 1具有典型的欧美B亚型GPGR序列 ,sz8- 2为GPGH ,其它毒株均为泰国B(B′)和E亚型所特有的GPGQ序列 ;进一步系统树分析显示 ,sz1- 2、sz8- 1、sz46 - 1与Bcon丛集一起 ,sz1- 1、sz8- 2和sz46 - 1分别与Econ、Ccon丛集。结论 :同一个体可以同时感染不同亚型HIV - 1毒株 。
To investigate whether the combination infection with different HIV-1 subtypes existed in HIV-1 infected population in China,and to analyse their infection source,.Methods HIV-1 env gene was amplified by nested PCR from uncultured peripheral blood mononuclear cells(PBMCs) obtained from 3 HIV-1 seropositive individuals found in Shenzhen.The C2-V3 region(360bp) of them was sequenced and analyzed.Results Of 3 HIV-1 infectors,2(sz1 and sz8)were infected with subtype Thai B(B′) and E with 23.52% and 20.69% intersubtype gene divergence inside individual,one subtype Europe and America B and C with 28.44%intersubtype divergence.In comparison of Bcon,Ccon,Econ HIV-1 internatiional reference sequence with corresponding subtypes found in these 3 infectors,the divergence waw 5.07-7.26%,3.23% and 5.38-5.99%,respectively.The tetra peptide in the crown of gp120 V3 loop found in sz46 who was a drug user and prostitute was GPGR characterized for Europe and America B subtypes,others were GPGQ characterized for Thai B and E.Phylogenetic tree analysis further shown that sz1-2,sz8-1,sz46-1 were tightly clustered with international Bcon,sz46-2 with Ccon,sz1-1 and sz8-2 with Econ,respectively.Conclusion These findings suggested that 3 individuals were infected with two different HIV-1 subtype probably result from multirisky behaviors.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2000年第4期333-335,共3页
Chinese Journal of Public Health
关键词
艾滋病毒
混合感染
分子流行病学
HIV-1 Combination infection env gene Sequence analysis
