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雷帕霉素和紫杉醇对骨髓内皮祖细胞数量及功能的影响 被引量：4

Effects of Rapamycin and Paclitaxel on the Number and Activity of Bone Marrow-Derived Endothelial Progenitor Cells
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摘要 目的:研究雷帕霉素和紫杉醇对骨髓内皮祖细胞(endothelial progenitor cells,EPCs)数量及功能的影响。方法:将离心法获得的SD雄性大鼠的骨髓单个核细胞接种于明胶包被的培养板上,培养7 d后收集贴壁细胞,加入不同浓度的雷帕霉素(0.1μg/mL,1μg/mL和5μg/mL)及紫杉醇(0.1μg/mL,1μg/mL和5μg/mL)培养24 h。在激光共聚焦显微镜下观察细胞,DiI标记的乙酰化低密度脂蛋白(DiI-acLDL)和异硫氰酸荧光素标记的单叶豆凝集素1(FITC-BS-1 Lectin)双染色阳性的细胞为正在分化的EPCs,在倒置荧光显微镜下对其进行计数。然后分别采用四甲基偶氮唑盐(MTT)比色法、改良的Boyden小室法和黏附能力检测实验来观察EPCs的增殖能力、迁移能力及黏附能力。结果:EPCs数量随着雷帕霉素及紫杉醇浓度的增加而减少,其增殖能力、迁移能力及黏附能力亦随药物浓度的增加而降低,各项指标有统计学意义(P<0.05)。相同浓度的雷帕霉素和紫杉醇对EPCs数量及功能的影响无显著差异。结论:雷帕霉素和紫杉醇可降低EPCs的数量及其增殖能力、迁移能力和黏附能力,且两种药物对EPCs的效应呈浓度依赖性。 Objective：To investigate the effects of rapamycin and paclitaxel on the number and activity of bone marrow-derived endothelial progenitor cells（EPCs）. Methods：Bone marrow mononuclear cells were isolated from rat bone marrow by centrifugation and they were cultured on gelatin-coated dishes. After 7-day culture, adherent cells were treated with rapamycin or pa- clitaxel in a series of concentrations （0.1 μg/mL, 1μg/mL, and 5μg/mL） for 24 h. EPCs were identified as adherent cells doub le positive for DiI-acLDL uptake and lectin binding by direct fluorescent staining. Proliferation and migration of EPCs were de termined using MTT assay and modified version of the Boyden chamber assay, respectively. EPCs adhesion assay was per formed by replanting the cells on gelatimcoated dishes, and then adherent cells were counted. Results： Incubation of EPCs with rapamycin or paclitaxel decreased the number of EPCs. Rapamycin and paclitaxel also decreased the proliferative, migratory, and adhensive capacity of EPCs in a concentration-dependent manner（P〈0.05）. At the same time, the influence of the same concentrations of rapamycin and paclitaxel on the number and function of EPCs showed no significant differences. Conclusions： Rapamycin and paclitaxel decreased the number, as well as the proliferative, migratory, and adhensive capacity of EPCs.
作者 朱琳琳 陈绍良 张娟 刘志忠
机构地区 南京医科大学附属南京第一医院心内科
出处 《中国临床医学》 2012年第5期459-461,共3页 Chinese Journal of Clinical Medicine
基金 江苏省自然科学基金(编号:BK2009044)
关键词 雷帕霉素 紫杉醇 内皮祖细胞 细胞培养 Rapamycin Paclitaxel Endothelial progenitor cells Cell culture
分类号 R979.1 [医药卫生—药品]
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参考文献8

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同被引文献30

  • 1梅审之,刘秋芳,谭丽,孙英.86例胸部肿瘤放射治疗心电图改变[J].现代肿瘤医学,2005,13(3):410-411. 被引量：10
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  • 3David Hassel,Paul Cheng,Mark P, et al . MicroRNA 10 reg- ulates the angiogenic behavior of zebrafish and human endo- thelial cells by promoting vascular endothelial growth factor signaling[J]. Grc Res ,2012,111(11) :1421-1433.
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  • 7FA Stewart.Mechanisms and dose-response relationships for radiation-induced cardiovascular disease[J]. Annals of the ICRP, 2012, 41(3-4) :72-79.
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  • 9Lauren MT,Joshua EM,Erin McSpadden,et al.Women at increased risk for cardiac toxicity following chemoradia- tion therapy for esophageal carcinoma [J]. Practical Radia-tion Ontology ,2013,3(4) : 149-155.
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中国临床医学
2012年 第5期

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