摘要
为建立一种快速的新城疫病毒病原检测方法,根据GenBank上登录的NDV F基因序列,利用生物学软件设计合成一对特异性引物,通过反应条件的优化,建立了检测NDV的RT-PCR一步法。该方法对传染性支气管炎病毒、传染性法氏囊病病毒、传染性喉气管炎病毒的扩增结果均为阴性,检测的敏感性达1ngRNA。临床初步应用显示该检测方法特异性强、敏感性高,可以用于新城疫病毒的临床快速检测。
To establish a rapid Newcastle disease virus （NDV） detection method, based on published NDV F genome sequence in GenBank, one pairs of primers were synthesized. Through optimization of the reaction conditions, a one step RT-PCR for detection NDV was established. The method used for infectious bronchitis virus（IBV）, infectious bursaldisease virus （IBDV）, infectious laryngotracheitis virus （ILTV）, all of the PCR results were negative~ the sensitivity was 1 ng RNA. Preliminary clinical application showd that the method has strong specificity, high sensitivity, and can be used for the rapid detection of Newcastle disease virus.
出处
《动物医学进展》
CSCD
北大核心
2012年第12期141-144,共4页
Progress In Veterinary Medicine
