摘要
目的通过测定胃血止糊剂中大黄素和大黄酚含量,为控制该制剂质量提供相关指标和方法。方法用高效液相色谱(HPLC)建立测定大黄中大黄素和大黄酚含量的方法。结果大黄素和大黄酚的平均回收率为97.56%,98.81%,变异系数(RSD)分别为1.27%,1.47%。大黄素的回归方程为y=2 842.3x-4.159 1(r=0.999 9),线性范围为0.009 2～0.184μg;大黄酚的回归方程为y=4 080x-5.985 3(r=0.999 8),线性范围为0.020 8～0.416μg。结论本文建立的HPLC法测定大黄素和大黄酚在胃血止糊剂中的含量,简便易行,重现性好,具有一定的专属性,可作为该药质量控制的指标和方法。
Objective To determine the content of arehin and chrysophanol in gastric blood paste,by which provide the indexes for evaluating the preparation quality control. Methods To establish the method of determination of arehin and chrysophanol in rhubarb by high performance liquid chromatography （ HPLC ）. Results The average recovery of Emodin or Chrysophanol was 97.56% ,98.81% , and the relative standard deviation （RSD） was 1.27 %, 1,47 % , respec- tively. The regression equation of archin is y = 2 842.3x - 4.159 1 （ r = 0. 999 9 ）, the linear range is 0.009 2 ～0.184 μg. The regression equation of chrysophanol is y = 4 080x - 5. 985 3 （ r = 0. 999 8 ） , the linear range is O. 020 8 ～ 0, 416 μg. Conclusions The HPLC method set for determination of Archin and Chrysophanol in Bastrie Blood Paste in this study is Simple, good for repetition and Specialization, which could provide the indexes for evaluating the preparation quality control.
出处
《中南医学科学杂志》
CAS
2012年第6期616-620,共5页
Medical Science Journal of Central South China
基金
云南省卫生厅院内制剂专项资助项目-云财社[2005]198号
