摘要
目的:探讨汉族人群中血管紧张素转换酶抑制剂(ACEI)所致咳嗽与血管紧张素转换酶(ACE)基因及缓激肽β2受体(BDK-RB2)基因多态性的关系。方法:应用聚合酶链反应(PCR)方法,检测汉族人群中151例由于服用ACEI引起的咳嗽患者及151例未发生咳嗽的患者的ACE I/D及BDKRB2 C/T的多态性,并采用紫外法检测ACE活性。结果:发现ACE基因分布在咳嗽组中II型为47.0%,ID型为42.4%,DD型为10.6%;无咳嗽组分别为39.7%、47.0%、13.3%,两组相比其差异具有统计学意义(P<0.01);BDKRB2基因分布在咳嗽组中CC型为21.3%,CT型为50.0%,TT型为28.7%,无咳嗽组分别为22.5%、47.7%、29.8%,两组相比其差异无统计学意义(P>0.05);咳嗽组ACE活性水平为([28.3±10.1)U/L]明显低于无咳嗽组([40.2±9.4)U/L],两组相比其差异具有统计学意义(P<0.01)。结论:汉族人群中ACEI所致咳嗽与ACE基因多态性及血清ACE水平有关,BDKRB2 C/T与咳嗽间未发现有统计学意义的关联。
Objective： To investigate the association between angiotensin converting enzyme inhibitor （ACEI）-induced cough and the polymorphism of angiotensin converting enzyme （ACE） gene and bradykinin 62 receptor （BDKRB2） gene in Hart nationality. Methods： Polymerase chain reaction （PCR） was used to examine ACE I/D and BDKRB2 CT polymorphism in 151 ACEI-induced cough subjects and 151 no cough subjects in Han population. And UV-method was used to detect the ACE activity. Results：ACE gene distribution in the cough group： type II was 47.0%, ID genotype was 42.4%, DD genotype was 10.6%; Non-cough group were 39.7%, 47.0%, 13.3% respectively, and there was statistically significant difference between the two groups（P〈0.01）; BDKRB2 Gene distribution in the cough group： the CC genotype was 21.3%, CT genotype was 50.0%, TT genotype was 28.7%, non-cough group were 22.5%, 47.7%, 29.8% respectively, and there was not statistically significant between the two groups （ P〉 0.05）; The level of ACE activity in the Cough group [（28.3 ± 10.1） U / L,] was significantly lower than non-cough group [（40.2 ± 9.4） U / L,（P 〈0.01）.]. Conclusions： For ban population, ACEI-induced cough related to ACE gene polymorphism and serum ACE activity, and there was no statistically significant association between BDKRB2 C / T and cough.
出处
《现代生物医学进展》
CAS
2012年第31期6011-6014,共4页
Progress in Modern Biomedicine
基金
湖南省科技厅重点项目资助(S2009S2032)
