摘要
建立一种正相高效液相色谱法测定鱼肝油中VD3含量的新方法。采用Agilent ZORBAX Rx-SIL色谱柱,以95%正己烷-5%异丙醇为流动相,流速为1.0 mL/min,PDA检测波长264 nm。结果表明:VD3浓度在0.2μg/mL～20μg/mL范围内,峰面积与含量具有良好的线性关系(r=0.999 7);平均相对标准偏差为0.62%,平均回收率为94.2%。该方法可作为鱼肝油中VD3含量的一种快速检测方法。
A rapid detection method of vitamin D3 in cod liver oil by NP-HPLC was established. The sample was determined under the following conditions： ZORBAX Rx-SIL column,95 % normal hexane phosphate-5 % isopropanol at 1.0 mL/min, and PDA detection at 264 nm. The results showed that a good linear relationship with in the eoneentration of 0.2 μg/mL-20 μg/mL （r=0.999 7）. The average relative standard deviation was 0.62 %, and the average recovery of added standard sample was 94.2 %. It can be used for vitamin D3 in cod liver oil.
出处
《食品研究与开发》
CAS
北大核心
2012年第11期173-175,共3页
Food Research and Development
关键词
正相高效液相色谱
二极管阵列检测器
维生素D3
normal phase high performance liquid chromatography
photo-Diode Array Detetor
vitamin D3
