摘要
根据猪肺炎支原体(Mhp)和猪鼻支原体(Mhr)的16S rRNA基因设计3条引物,建立Mhp和Mhr的双重PCR检测方法,并对该方法进行了特异性和敏感性试验。应用建立的方法检测了临床样品和疫苗样品。结果显示,该方法具有良好的特异性,最低可检测到0.66 ng的Mhp基因组DNA和0.58 ng Mhr基因组DNA。临床样品和疫苗样品检测结果与普通PCR检测结果一致。该双重PCR方法可用于Mhp与Mhr的鉴别、诊断以及疫苗纯粹性检查,快速而准确。
According to the 16S rRNA gene of Mycoplasma hyopneumoniae（Mhp） and Mycoplasma hyorhinis（Mhr）,we designed three primers and established a double PCR method for simultaneous detecting Mhp and Mhr,and the specificity and sensibility assay of the method were performed.The clinical samples and vaccine sample were detected by the method.The results showed that the specific DNA fragment could be amplified from Mhp and Mhr,and the minimum detection limit could reach to 0.66 ng of Mhp genome DNA and 0.58 ng of Mhr genome DNA.The results of clinical samples and vaccine samples test with the double PCR were consisted with that by the normal PCR.Therefore,the double PCR method is a fast and accurate method for the detection and diagnosis of Mhp and Mhr.
出处
《中国兽药杂志》
2012年第9期7-10,共4页
Chinese Journal of Veterinary Drug
基金
国家自然科学基金(31100136)
江苏省农业科技自主创新资金项目[CX(11)3025]
关键词
猪肺炎支原体
猪鼻支原体
PCR
检测
Mycoplasma hyopneumoniae
Mycoplasma hyorhinis
PCR
Detection
