摘要
目的 探讨沙眼衣原体 (CT)所致新生儿结膜炎的流行病学情况。方法 采用细胞培养和PCR两种方法检测 1997年 10月～ 1998年 9月重庆医科大学附属儿童医院 12 5例新生儿结膜炎患儿的结膜标本中的CT。结果 CT感染阳性率为 5 1.2 % (6 4/ 12 5 ) ,经阴道分娩儿CT感染率 (74% ,6 0 / 81)明显高于剖宫产儿 (9% ,4/ 44 ) (χ2 =48.2 0 ,P <0 .0 1) ;细胞培养和PCR对结膜标本CT的检出率分别为 41.6 % (5 2 / 12 5 )和 48.8% (6 1/ 12 5 ) ,敏感性分别为 81.3%和 94.2 % ,二者差异有显著性(χ2 =70 .47,P <0 .0 5 )。结论 该组新生儿结膜炎的最主要病原菌是CT ;新生儿CT感染与分娩方式有一定的关系 ,产道是新生儿获得CT感染的主要途径。但是 ,宫内CT感染也可引起新生儿结膜炎。对CT的检测 ,PCR的敏感性高于细胞培养。
Objective To investigate the prevalence of neonatal conjunctivitis caused by Chlamydia trachomatis and to evaluate two methods of detecting Chlamydia(C.) trachomatis in neonatal conjunctival specimens. Methods A total of 125 conjunctival scrapings taken from neonates with conjunctivitis in Children′s Hospital during October 1997-September 1998 was tested by the McCoy cell culture and polymerase chain reaction (PCR). Results The detection rates of C. trachomatis in neonatal conjunctivitis were 41.6% by cell culture and 51.2% by PCR, respectively. The sensitivities of cell culture and PCR were 81.3% and 94.2%, respectively. The prevalence of C. trachomatis conjunctivitis was obviously higher in neonates delivered through vagina than those by cesarean section delivery (74% and 9%, respectively, P <0.01). Conclusion C. trachomatis was the main pathogenic microorganism of neonatal conjunctivitis in Children′s Hospital during the year 1997 to 1998 (51.2%). The C. trachomatis infection in neonates related to the delivery mode, espevially the passage through the birth canal, but sometimes may be caused by the intrauterine infection. PCR showed more sensitive than the cell culture in the detection of C. trachomatis.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2000年第6期377-379,共3页
Chinese Journal of Pediatrics
关键词
沙眼衣原体结膜炎
新生儿
流行病学
Chlamydia trachomatis
Infant, newborn
Conjunctivitis, bacterial
Cells, cultured
Polymerase chain reaction
