摘要
所构建的杜仲果实和叶片转录组数据库获得了54 471 338条reads数据,包含4 902 420 420nt数据信息,对reads进行拼接,获得了452 421条Contig,总长度为90 705 736 nt;对contig进行拼接后,获得了147 027条Scaffold,总长度为65 877 052 nt;同理,对Scaffold进一步拼接后,获得了49 610条Unigene,总长度为37616 729 nt;Unigene和COG数据库进行比对表明,杜仲果实和叶片转录组中的Unigene根据功能大致可分为25类;杜仲果实和叶片转录组中的Unigene根据GO功能可分为生物过程、细胞组分和分子功能3大类42分支;以KEGG数据库为参考,依据代谢通路可以将转录组中的数据分成125类,包括脂类代谢,DNA剪切,植物激素生物合成,苯丙氨酸生物合成,萜类化合物与类固醇类化合物合成等。
The transcriptome library of Eucommia fruits and leaves contained 54471338 reads consist of 4902420420 nt. 452421 Contigs were obtained by assembling the reads, and the total length of all Contigs was 90705736 nt;In the transcriptome library, 147027 Scaffolds were obtained by assembling the Contigs, and the total length of all Scaffolds was 6587705 nt; Also the 49610 Unigenes were obtained by by assembling the Scaffolds in the transcriptome library, and the total length of all Unigenes was 37616729 nt;Unigene and the COG database to compare, Unigene in the transcriptome of Eucommia fruits and leaves can be broadly divided into 25 classes according to the function; The Unigenes GO functions in the the transcriptome library were classificated into 3 categories： biological process, cellular component and molecular function and 42 branches;In this study, the KEGG database as a reference,data in the transcriptome can be divided into 119 classes,according to the metabolic pathway, such as lipid metabolism, DNA replication, biosynthesis of plant hormones, Biosynthesis of phenylpropanoids, Biosynthesis of terpenoids and steroids and so on.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2012年第11期122-130,共9页
Journal of Central South University of Forestry & Technology
基金
国家林业公益性行业科研专项(201004029)
国家十二五科技支撑计划(2012BAD21B0502)
关键词
杜仲
果实
叶片
转录组
基因功能
Eucommia ulmoides
fruits
leaves
transcriptome
Gene Ontology
