摘要
目的观察异硫氰酸萘酯(ANIT)诱导的急性肝内胆汁淤积大鼠肝组织法尼醇受体(FXR)的表达的变化及意义。方法雄性SD大鼠随机分为正常组8只,模型组24只,模型组采用4%ANIT(麻油配制)100mg/kg一次性灌服诱导大鼠肝内胆汁淤积,于给药后24、48、72h 3个时相点随机处死8只大鼠,正常组以等容量麻油一次性灌胃后48h处死;生化法检测血清TBA水平,荧光定量PCR检测肝组织FXRmRNA的表达,免疫组化法检测FXR蛋白的表达。结果模型组大鼠血清TBA水平在灌服ANIT 24h后明显升高并达高峰,之后逐渐下降,3个时间点均较正常组明显升高;而肝组织FXRmRNA和蛋白表达在3个时间点均较正常组明显下降,其中24h最低,随时间推移,48、72h逐渐增高;相关性分析发现,大鼠肝组织FXRmRNA表达与血清TBA水平均呈明显的负相关(r=-0.589,P<0.01)。结论大鼠肝组织FXRmRNA和蛋白表达的下降可能参与ANIT诱导的急性肝内胆汁淤积病理过程。
Objective To investigate the expression of farnesoid X receptor in the liver issue of rats with acute intrahepatic cholesta- sis and its significance. Methods Male SD rats were randomly divided into 2 groups,with 8 rats in the normal group,24 rats in the mod- el. The model of acute intrahepatic cholestasis in rat was induced by 4% ANIT prepared with sesame oil by gastric gavage. At the ends of 24th ,48th and 72th hour after the administration of ANIT,8 rats in model group were respectively randomly sacrificed. The rats in the normal group were administrated with sesame oil by gastric gavage and sacrificed at the 48th hour. The serum TBA level of the rats were detected by full automatic biochemical analyzer. The expression of FXRmRNA in liver were measured by RT - PCR. The protein expression of FXR were detected by immunohistochemistry. Results The serum TBA level was significantly increased in the model group at all three time points. In the 24 hour after administration of ANIT,the serum TBA level was in creased and reaohed the peak time, and then it was de- creased. At the same time the protein and mRNA expression of FXR were significantly decreased in the model group at all three time points. The expression of FXRmRNA in liver and the serum TBA level had significantly negative correlation （ r = - 0. 589, P 〈 0.01 ） by correlation analysis. Conclusion The decrease of the protein and mRNA expression of FXR in the rats liver may participate in the patho- logic process of acute intrahepatic cholestasis induced by ANIT.
出处
《医学研究杂志》
2012年第12期173-175,共3页
Journal of Medical Research
关键词
法尼醇受体
肝内胆汁淤积
Farnesoid X receptor
Intrahepatic cholestasis
