摘要
目的:建立密脉鹅掌柴提取物中总皂苷及齐墩果酸的含量测定方法。方法:总皂苷的含量测定采用比色法,于552nm测定;齐墩果酸的含量测定采用高效液相色谱法,色谱柱:Agilent ZORBAX SB-C_(18)(250 mm×4.6 mm,5μm),流动相:乙腈-水(84:16),流速:1.0 ml·min^(-1),检测波长:203 nm,柱温:25℃。结果:按选定的紫外条件进行测定,齐墩果酸在2.0～20.0μg·ml^(-1)范围内呈良好线性关系(r=0.999 5),平均回收率为99.57%;HPLC定量试验中,齐墩果酸在0.4～8.0μg范围内呈良好线性关系(r=0.999 9),平均回收率为100.44%。结论:建立的方法简便易行,结果准确可靠,可作为本产品的质量控制方法。
Objective：To establish the method for determination of total saponins and oleanolic acid in Schefflera vemdosa extracts. Method：The content of total saponins was detennined by UV at 552 nm.The separation and determination of oleanolic acid were performed on an Agilent ZORBAX SB-C_（18）（250 mm×4.6 mm,5μm） column with the mobile phase consisted of acetonitrile-water （84：16） at the flow rate of 1.0 ml ？ min^（-1）,the detection wavelength was 203 nm,and the column temperature was 25X1.Result： The standard curve was linear within the range of 2.0-20.0μg ？ ml^（-1） for oleanolic acid of UV,0.4-8.0 u,g for oleanolic acid of HPLC.The average recovery of total saponins and oleanolic：acid was 99.57%and 100.44%,respectively.Conclusion：The developed methods are rapid and accurate with high repeatability and stability,and can be used in the quality control of Schefflera venulosa extracts.
出处
《中国药师》
CAS
2012年第11期1551-1553,共3页
China Pharmacist
基金
云南省科研院所技术开发研究专项重点项目(编号:2006KFZX-01)
关键词
密脉鹅掌柴提取物
总皂苷
齐墩果酸
含量测定
Schefflera venulosa extracts
Total saponins
Oleanolic acid
Determination
