摘要
以罗布麻叶中内生真菌为研究对象,用化学显色法和薄层层析显色法对产生黄酮的内生真菌进行筛选,并根据形态特征对筛选到的内生真菌进行鉴定。结果表明:内生真菌菌株P-1和P-2均能产生黄酮;菌株P-1为镰孢菌(Fusariumsp.),菌株P-2为嗜松青霉(Penicillin pinophilum)。
Plant endophytic microorganisms have a close metabolic relationship with its host plants. Endophytic fungi in leaves of Apocynum venetum was used as research material. Endophytic fungi with the capability of producing flavonoids were screened with the methods of chemical colorimetry and thin layer chromatography, and then they were identificated by observing their morphological characteristics. The results showed that two fungal strains named P-1 and P-2 were screened out. They were detected to be able to produce flavonoid compounds. Strain P-1 belongs to Fusariurn,whereas strain P-2 belongs to Penicillin pinophilum. This study laid a foundation for further exploitation of the endophytic fungi in A. venetum.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2013年第1期59-62,共4页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(30960227)
关键词
罗布麻
内生真菌
黄酮
筛选
鉴定
Apocynum venetum
endophytic fungi
flavonoids
screening
identification
