期刊文献+

DNA改组——定向进化的技术革命 被引量:4

DNA Shuffling——technological revolution of directed evolution
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摘要 DNA改组 (DNAshuffling)是 90年代中期发展起来的一种新技术。该文概述了有关DNA改组的基本原理和方法 ,并跟踪了当今该领域的最新研究成果和发展动态。 Developed in the middle of 90′s, DNA shuffling has attracted closed attention of people since its birthday. Recently, this technique is being employed by more and more biotechnological engineering labs and many significant results have been achieved. In this paper, the authors review the basic method and novel strategy of DNA shuffling, and follow the update development of this pioneering technique. [
出处 《军事医学科学院院刊》 CSCD 北大核心 2000年第2期130-134,共5页 Bulletin of the Academy of Military Medical Sciences
关键词 DNA改组 定向进化 基因表达 技术革命 DNA shuffling directed evolution gene expression technical revolution
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参考文献5

  • 1http :/ /www .maxygen .com/nf_research .html,1999年
  • 2Chang C C,Nature Biotechnol,1999年,17卷,8期,793页
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  • 4Wan L,Proc Nat Acad Sci SUA,1998年,95卷,22期,12825页
  • 5Zhang J H,Proc Nat Acad Sci SUA,1997年,94卷,9期,4504页

同被引文献18

  • 1Stemmer WPC.DNA shuffling by random fragmentation and reassembly:In vitro recombination for molecular evolution.Proc Natl Acad Sci USA,1994,91 (22):10747~10751
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  • 6Muller KM,Stebel SC,Knall S,et al.Nucleotide exchange and excision technology (NExT) DNA shuffling:a robust method for DNA fragmentation and directed evolution.Nucleic Acids Res,2005,33(13):e117
  • 7Crameri A,Raillard SA,Bermudez E,et al.DNA shuffling of a family of genes from diverse species accelerates directed evolution.Nature,1998,391 (6664):288~291
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  • 10雷燕.浅谈分子生物学技术在中医药研究领域中的应用[J].北京中医药大学学报,1998,21(6):27-30. 被引量:8

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