摘要
目的 观察日本血吸虫单克隆抗独特型抗体NP30主动免疫对山羊的保护性免疫作用。方法 将 2 2只山羊随机分为两组 :(1)实验组 :12只 ,每只山羊NP30的免疫剂量为 10 0 0 μg/次 ,后腿肌肉注射 ,连续免疫 3次 ,最后 1次免疫后 8周进行尾蚴攻击。 (2 )对照组 :10只 ,肌肉注射SP2 / 0腹水。尾蚴攻击感染后第 12周处死。结果 NP30主动免疫山羊可诱导 42 .78%的减虫率 ,肝组织减卵率为 35 .83 % ,粪减卵率为 2 5 % ,并可明显抑制肝脏虫卵肉芽肿的大小 ,肉芽肿数量明显减少 ,体重增加。
Objective To observe the protective immunity in goats induced by active immunization with anti idiotypic monoclonal antibody NP30 of S.japonicum . Methods Goats were randomly divided into two groups. Experimental group had 12 goats. The NP30 was intramuscularly injected with a dosage of 1 000 μg into the hind leg for three dosages continuously. The goats were challenged with cercariae eight weeks after the third immunization. Ten goats in the control group were intramuscularly injected with SP2/0 ascites and challenged with cercariae at the same time as the experimental group. All goats were killed at the twelfth week post challenge. Results Active immunization with NP30 could produce a protection rate of 42.78% in goats. The number of eggs in liver and feces in the goats immunized with NP30 was decreased by 35.83% and 25%, respectively. The size and number of egg granulomas in the liver were lessened obviously and the weight of the goats increased significantly. Conclusion Active immunization with NP30 could induce a good protection in goats against the infection with Schsitosoma cercariae.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2000年第6期411-413,共3页
National Medical Journal of China
基金
江苏省科委社会发展基金资助项目!(BS97036)
