摘要
本研究以果蝇为模式生物,应用果蝇全基因组表达谱芯片技术研究刺五加对果蝇睡眠-觉醒相关基因表达的影响,并通过生物信息学中数据分析技术探讨刺五加改善睡眠的机制。研究结果表明,刺五加改善果蝇睡眠的作用是以dco、slo、Nos和DopR2等基因为主要参与者的多基因参与的过程和多基因共同调节的结果。
In this study, sleep-deprivation drosophila models were made by the alternative light-dark stimulating at night. On this basis, the acting mechanism was explored by using the mieroarray technology of whole-genome of drosophila and bioinformatics data analysis techniques. The results indicated that Acanthopanax senticosus has effect on the sleep improvement of drosophila models through the co-regulation of many genes, such as dco, slo, Nos and DopR2.
出处
《世界科学技术-中医药现代化》
北大核心
2012年第6期2170-2177,共8页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金项目(81073077):四逆散有效组分的改善睡眠作用机理与体内代谢过程研究
负责人:李廷利
福建省自然科学基金青年创新项目(2011D016):刺五加治疗失眠的药效物质基础及作用机制研究
负责人:许光辉
关键词
刺五加
果蝇
睡眠
基因芯片
Acanthopanax senticosus, Drosophila melanogaster, sleep, Genechip
