摘要
目的探讨儿童特应性皮炎血清和CD4＋CD25＋T分泌细胞因子IL-10表达，分析其与病程及严重程度的相关陛。方法特应性皮炎患儿按SCORAD指数分3组，轻度10例、中度16例、重度20例。抽取46例特应性皮炎患儿和31例健康对照儿外周血，用免疫磁珠分离获得CD4＋CD25＋T细胞，用ELISA法分别检测患病组和健康对照组血清及CD4＋CD25＋F细胞培养液IL-10水平，并分析IL-10水平与SCORAD评分的相关陛。结果轻、中、重度AD各组血清IL-10水平分别为（43．10±25．07）、（68．40±36．65）、（55．55±41．97）pg/Cml，与健康对照组（58．27±36．84）pg／ml比较，差异均无统计学意义（P〉0．05），与患儿SCORAD评分无相关性。轻、中、重度AD组CD4＋CD25＋T细胞分泌IL-10含量分别为（52．96±11．69）、（49．86±9．18）、（27．25±7．01）pg／ml，重度AD组低于健康对照组（55．15±11.15）pg／ml（P〈0．05）；轻、中度组与健康对照组差异无统计学意义（P〉0．05）。CD4＋CD25＋T细胞分泌IL-10的水平与患儿疾病严重程度SCORAD评分呈明显负相关（r值分别为-0．757，P〈0．01）。结论CD4＋CD25＋T细胞及相关因子IL-10可能参与儿童特应性皮炎的发病。
Objective To determine the level of interleukin （IL）-10 in sera and culture supernatants of CD4=CD25=T cells from children with atopic dermatitis （AD）, and to evaluate its relationship with clinical course and severity of AD. Methods Totally, 46 children with AD and 31 healthy controls were included in the study. All the patients were divided into 3 groups, i.e., mild （n = 10）, moderate （n = 16） and severe （n = 20） group, according to severity scoring of atopic dermatitis （SCORAD） score. Venous blood samples were obtained from the patients and healthy controls. CD4＋CD25＋ regulatory T cells were separated from the blood samples by magnetic cell sorting （MACS） system in two steps and cultured in vitro. Enzyme linked immunosorbent assay （ELISA） was conducted to quantify the level of IL-10 in sera and culture supematants of CD4＋CD25 ＋ T cells from these subjects. Analysis of variance was carried out to compare the level of supernatant and serum IL-10 between the patients and controls, and Pearson correlation analysis to assess the relationship between the level of IL-10 and SCORAD score. Results The patients with mild, moderate and severe AD showed a similar serum IL-10 level compared with the healthy controls （（43.10 ± 25.07） pg/ml, （68.40± 36.65） pg/ml and （55.55 ± 41.97） pg/ml vs. （58.27 ± 36.84） pg/ml, all P 〉 0.05）. The level of supernatant IL-10 secreted by CD4＋CD25＋ T cells from the controls was significantly higher than that from the patients with severe AD （（55.15 ± 11.15） pg/ml vs. （27.25 ± 7.01 ） pg/ml, P 〈 0.05）, but similar to that from the patients with mild and moderate AD （ （52.96 ± 11.69） pg/ml and （49.86± 9.18） pg/ml, respectively, both P 〉 0.05） . The level of secreted IL-10 was negatively correlated with SCORAD score （r = -0.757, P 〈 0.01 ）, whereas the serum level of IL-10 showed no statistical correlation with SCORAD score. Conclusion CD4＋CD25＋ T cells and IL-10 may be implicated in the development of AD.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2013年第1期13-15,共3页
Chinese Journal of Dermatology
基金
浙江省温州市科技局对外合作课题（H20100016）
