摘要
对2个猪场的母猪血清、扁桃体、全血样品和公猪精液分别应用荧光抗体染色法、RT-PCR、CS-FV抗原ELISA、CSFV糖蛋白Erns ELISA 4种方法进行CSFV对应检测和比较。结果显示,荧光抗体染色法检出率最高,但与其他3种方法存在较大差异,RT-PCR方法检出率和重复性次之;猪瘟抗原ELISA方法检出率第3,重复性第1;CSFV糖蛋白Erns检出率和重复性均最低,但可筛选是否感染猪瘟病毒野毒。在母猪3种样品中扁桃体的检出率最高,全血样品次之,血清检出率最低。4种样品中公猪精液重复性最好,其次是母猪全血样品,母猪扁桃体第3;母猪血清样品最差。
Detection of CSFV the samples of sera, tonsil, whole blood and semen from two pig farms were detected respectively by fluorescent antibody dying（DFA）, RT-PCR, CSFV antigen ELISA and CSFV glycoprotein Erns （gp44/48）ELISA kit. The results showed that the fluorescent antibody dying technique got the highest sensitivity in one of 4 methods, but there was large difference with the other three methods, followed by RT-PCR method of detection rates and repetitiveness. Detection rate of CSFV ELISA methods was third, repeating the first. Detection rate of CSFV glycoprotein Ems ELISA and repeatability were the lowest, but which could determine the pigs infec- ted by classical swine fever virus. Among sow 3 kinds of samples, tonsil had the highest detection rate, followed by whole blood samples, the minimum detectable rate of serum. In 4 kinds of samples , boar semen were the best duplication, followed by the whole blood of sow, sow tonsils third and worst of sow sera.
出处
《动物医学进展》
CSCD
北大核心
2013年第1期66-71,共6页
Progress In Veterinary Medicine
基金
广西水产畜牧兽医局科研计划项目(桂渔牧科061911)
关键词
猪瘟病毒
扁桃体
全血
血清
精液
检测方法
Classical swine fever virus
tonsil
whole blood
serum
semen
detection methods
