摘要
为了探索一种简便易行、具有良好应用性并适用于中医药领域免疫功能低下的动物模型,以为福建黄兔提供一种新型实验动物的实验依据。实验采用氢化可的松为细胞免疫抑制剂,6个实验组,其中1个对照组,5个剂量组(分别每天20、25、30、35、40mg/kg),采用流式细胞检测法测CD4、CD8细胞含量,并通过摘取脾脏的方式计算脾脏指数。实验结果显示:B、C剂量组(分别每天25、30mg/kg)的福建黄兔在注射药物前后CD4、CD8细胞含量呈现明显的下降趋势,证实上述2个剂量组是福建黄兔免疫功能低下模型造模的较好剂量;同时,A～E剂量组的黄兔脾脏相较于对照组均呈现不同程度的缩小,脾脏指数降低,B、C、D组与对照组差异显著,反映其免疫功能受损,其中尤以C剂量组(30mg/kg)下降最为明显。由此可见,C剂量组是本实验中免疫功能低下动物模型造模的最适宜剂量。
In order to develop an immunosuppression animal model with easy replication and good economic application which also applicable in the field of traditional Chinese medical research. At the same time, it provided a new kind of experimental basis for Fujian Rabbit. The experiment took the hydrocortisone as injection, 6 experimental groups, 1 control group and five dosage groups （at 20, 25, 30, 35, 40 mg/kg daily respectively）, CD4, CD8 cell content would be examined by flow cytometry and spleen was removed in order to calculate the spleen index. The results showed that, after injection, CD4 and CD8 cell in B, C dosage group （25, 30 mg/kg respectively） had gone down obviously which demonstrated that they were the most appropriate injection dosage. There were different degrees of reduction in weighted spleen of dosage groups when compared with the control group, which implied the immunity function had damaged. Then, B, C, D group showed a more significant level of decline, especially with group C. It confirmed that the C group was the most appropriate injection dosage in immunosuppression animal model from this research.
出处
《中国农学通报》
CSCD
2012年第35期65-68,共4页
Chinese Agricultural Science Bulletin
基金
福建中医药大学校管课题"福建黄兔免疫抑制模型的建立"(X2011017)
