摘要
从云南松毛虫肠道中分离筛选得到28株产纤维素酶的菌株。经生理生化试验观察和16S rRNA序列比对分析,初步鉴定这些产酶菌株属于肠杆菌属、芽孢杆菌属、克雷伯氏杆菌属和约克氏菌属。同时筛选出一株产酶能力较高的菌株DK4,鉴定为蜡状芽孢杆菌,并构建系统发育树。对该菌株产酶特性进行了初步研究,其酶反应的最适温度是35℃,最适pH值7.0,连续发酵70 h左右时纤维素酶活达到最高值19.24 U/mL。
Twenty-eight Cellulase-producing strains were isolated from intestinal canal of Dendrolimu houi.Basing on biochemical,physiological characterization and 16S rRNA sequences blasting,these strains were classified into Enterobacter sp.,Bacillus sp.,Klebsiella sp.and Yokenella sp..Strain DK4 was found as a higher-yield strain for cellulase in these strains.A phylogenetic tree was constructed with the published 16S rRNA sequences of relative bacteria species.In the phylogenetic tree,the strain DK4 was in the branch with the Bacillus cereus strain NAV-10（GU056811.1）.The highest cellulase activity of DK4 strain was 19.24 U/mL when grown at 35℃,pH 7.0 for 70 h.
出处
《华北农学报》
CSCD
北大核心
2012年第B12期254-258,共5页
Acta Agriculturae Boreali-Sinica
基金
国家"十一五"重大科技支撑计划课题基金项目(No.2008BAD95B09)
关键词
肠道细菌
云南松毛虫
纤维素酶
鉴定
酶学性质
Intestinal bacteria
Dendrolimu houi
Cellulase
Identification
Enzyme characterization
