摘要
为了解豆状带绦虫成虫、幼虫及虫卵的蛋白组分及其之间的差异,比较分析其免疫反应性,应用SDS-PAGE从豆状带绦虫不同发育阶段的虫体中分离出4～8条主要蛋白区带,分子质量为30.4～114.9ku。取人工感染豆状带绦虫犬自然排出的孕卵节片,虫卵计数后以不同数量虫卵感染家兔,定期采血,用间接ELISA法检测家兔的抗体变化情况。结果显示,家兔自感染后第2周抗体水平开始升高,第7周开始逐渐下降。以第7周感染家兔血清对13种虫体抗原进行的Western-blot分析显示,13种虫体抗原在59.4ku处均能被兔抗豆状囊尾蚴血清识别,羊脑多头蚴与豆状囊尾蚴共有4条相似带,分子质量分别为32.7、59.4、78.7、81.1ku,特异性抗原极相似,表明虫种之间存在着强烈的交叉反应,59.4ku蛋白带很有希望成为预防兔豆状囊尾蚴病的候选抗原。
To analyze the protein components and their immunogenicity in adult,larva and egg of Taenia pisiformis,4-8 protein bands ranging from 30.4 to 114.9 ku were observed in SDS-PAGE gel.Rabbits were infected orally with the eggs in gravid proglottid secreted from the dogs that were experimentally infected with different numbers of Taenia pisiformis.Circulating antibody form rabbits experimentally infected with Cysticercus pisiformis were monitored by ELISA weekly.The antibody titers were raising from week 2 post-infection and reached the peak on week 7 post-infection.Thirteen antigens with molecular weight of around 59.4 ku were recognized by positive serum against Cysticercus pisiformis in Western-blot.Presence of cross reactions was found between Coenurus cerebralis and Cysticercus pisiformis.The molecular weights of the characteristic antigens was 32.7 ku,59.4 ku,78.7 ku and 81.1 ku,respectively.The 59.4 ku protein is a promising vaccine candidate for cysticercosis pisiformis prevention.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2012年第12期1259-1263,共5页
Chinese Veterinary Science
基金
甘肃省农业生物技术研究与应用开发项目(GNSW-2010-01)
甘肃省科技支撑计划项目(1104NKCA082)
