摘要
目的:建立气滞胃痛颗粒及其制剂中白芍、枳壳、甘草的高效液相特征图谱分析方法。方法:采用HPLC-DAD法,以新橙皮苷为内参照物,采用依利特C18色谱柱(4.6 mm×150 mm,5μm),以乙腈(A)-水(B)为流动相梯度洗脱(0～45min,10%→28%A;45～50 min,28%→95%A),流速1.0 mL.min-1,柱温30℃。结果:气滞胃痛颗粒特征图谱中,共标记出10个与白芍、枳壳、甘草3种药材相对应的共有峰;对16个批次的气滞胃痛颗粒进行分析,相似度>0.90。结论:该方法简便、快速,可为气滞胃痛颗粒的质量提供更为科学、准确的控制。
Objective： To establish a chromatographic method for the analysis of Paeoniae Radix Alba, Aurantii Fructus and Glycyrrhizae Radix et Rhizoma in Qizhi Weitong particle. Method： Investigated by techniques of HPLC-DAD, Elite C18 column （4.6 mm×150 mm, 5 μm） was used. The mobile phase was acetonitrile-water （ 0-45 min, 10% -28 % A ; 45-50 min, 28 % -95 % A） at a flow rate of 1.0 mL ·min - 1. The column temperature was maintained at 30℃ Result： Measured value of sixteen batches revealed that there were ten common peaks with Paeoniae Radix Alba, Aurantii Fructus, Glyeyrrhizae Radix and high similarity between samples of specific chromatogram. Conclusion： The method is simple and accurate. It can provide a more scientific and accurate control for Qizhi Weitong particle.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第2期102-105,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
气滞胃痛颗粒
白芍
甘草
枳壳
特征图谱
HPLC-DAD
Qizhi Weitong particle
Paeoniae Radix Alba
Aurantii Fructus
Glycyrrhizae Radix etRhizoma
specific chromatogram
HPLC-DAD
