摘要
目的比较研究土人参多糖的酶法提取、超声辅助提取及2种方法联合提取的优劣。方法采用正交试验优化土人参多糖的酶法提取及超声提取方案,并研究2种方案的结合效果。结果3种提取工艺中,酶法提取多糖得率平均为17.45%,超声提取多糖得率平均为20.01%,酶-超声联合提取多糖得率平均为20.74%。结论酶-超声联合提取土人参多糖效果省时且高效。
Objective To comparatively study the advantage and disadvantage of different polysaecharide extraction methods of enzymatic extraction, ultrasonic extraction and a combination of these two processes. Methods The enzymatic extraction and ultrasonic extraction were first optimized by orthogonal design and then compared with the effect of combination. Results From the results of three extraction techniques, enzymatic extraction of Talinum crassifolium polysaccharide's average extraction rate was 17.45%, ultrasonic extraction was 20.01%, enzyme-ultrasonic extraction was 20.74%. Conclusion The combined optimum preparative procedure of Talinum crassifolium is simple, quickly and high efficient.
出处
《国际生物医学工程杂志》
CAS
2012年第6期353-356,共4页
International Journal of Biomedical Engineering
基金
2013年度自治区级大学生创新训练计划立项资助项目(QJCX201338)
关键词
土人参
多糖
纤维素酶
超声提取
正交设计
联合
Talinum crassifolium
Polysaecharide
Cellulase
Ultrasonic extracting
Orthogonal design
Combination