摘要
目的:建立RP-HPLC同时测定皮尔康胶囊中龙胆苦苷、黄芩苷、花椒毒素含量的分析方法。方法:采用DIONEX Ac-claim C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-水(14∶86)为流动相,流速1 mL·min-1,检测波长270 nm,柱温30℃。结果:龙胆苦苷、黄芩苷、花椒毒素进样量分别在0.10～1.5μg、0.032～0.47μg、0.0032～0.048μg范围内与峰面积呈良好的线性关系(r=0.9996～0.9999,n=5),平均回收率(n=6)分别为99.8%(RSD=0.76%)、100.2%(RSD=1.8%)、99.8%(RSD=1.9%)。结论:本法适用于皮尔康胶囊中龙胆苦苷、黄芩苷、花椒毒素3个成分的同时测定。
Objective： To develop a RP - HPLC method for the simultaneous determination of gentiamarin, baicalin and xanthotoxin in Pierkang capsules. Methods ： A DIONEX Acclaim C18 column （ 250 mm × 4.6 mm, 5 μm ） was used. The mobile phase was acetonitile -water（ 14： 86）at the flow rate of 1.0 mL .min -1,the detection wavelength was set at 270 urn, and the column temperature was 30 ℃. Results： There was a good linear relationship between the quantity and peak area in the range of 0. 10 - 1.5 μg,0. 032 -0.47 μg,0. 0032 -0. 048 μg for gentiamarin,baicalin and xanthotoxin（ r = 0. 9996 - 0. 9999, n = 5 ） , respectively ; the average recoveries of gentiamarin, baicalin and xanthotoxin were 99. 8% （ RSD = 0. 76% ）, 100. 2% （ RSD = 1.8% ）, and 99.8% （ RSD = 1.9% ）, respectively. Conclusion：The method is suitable for simultaneous determination of gentiamafin, baiealin and xanthotoxin in Pier- kang capsules.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2013年第1期116-119,共4页
Chinese Journal of Pharmaceutical Analysis
