摘要
[目的]优选室温下超声辅助提取仙人掌多糖的提取工艺.[方法]采用正交试验设计,分别考察液固比、超声时间、提取次数对提取率的影响.[结果]最佳提取工艺为:液固比35∶1 mL/g,超声时间25 min,提取次数2次.[结论]该工艺合理可行,可用于仙人掌多糖的提取.对多糖提取液的抗氧化研究表明,仙人掌多糖对羟自由基有一定的清除作用.
An orthogonal test was conducted in this study, with a view to optimizing the ultrasonic wave assisted extraction technology of polysaccharides from Opuntia at room temperature. The effects of material-water ratio, extrac- tion time and extraction frequence on the extraction efficiency of polysaccharides were studied. The results showed that the optimum extraction conditions were that the material-water ratio was 35:1 (mL/g), the extraction time was 2.5 min and the extraction frequence was twice. It was concluded that the optimal extraction technology was reasonable and suitable for the extraction of polysaccharides from Opuntia. The research on anti-oxidation properties showed that the extracted polysaccharides had a scavenging activity on hydroxyl free radical.
出处
《南京晓庄学院学报》
2012年第6期34-37,共4页
Journal of Nanjing Xiaozhuang University
关键词
仙人掌
多糖
正交试验
羟自由基
Opuntia
polysaccharides
orthogonal design
hydroxyl free radical