摘要
目的探讨放射线（6WV-X线）治疗骨包虫病的疗效。方法建立子午沙鼠骨细粒棘球蚴病动物模型72只，随机分成4组，1组作为对照组，另外3组分别给予40Gy／5次、50cy／5次、60Gy／5次，6WV-X线定位照射治疗，放疗结束3个月时观察子午沙鼠、囊液内头节死亡数、蛋白质及钙离子浓度变化；比较观察囊皮角质层、生发层病理组织学变化以及照射区域骨坏死；电镜下观察包虫囊角质层、生发层超微结构变化。结果各组均有死亡病例，但沙鼠一般行为正常。各组头节死亡数（分别为：对照组：22．6个，40Gy／5次组：96．6个，50Gy／5次组：131．5个，60Gy／5次组：155．5个）差异有统计学意义（P〈0．01）。囊液内蛋白质（钙离子）浓度随照射剂量升高而降低（升高），蛋白质（钙离子）浓度变化分别为对照组：1．0241（2．8875），40Gy／5次组：0．7245（3．0541），50Gy／5次组：0．6171（3．5446），60Cy／5次组：0．5058（3．8241），各照射组与对照组及各照射组之间比较差异有统计学意义（P〈0．01）。病理组织学显示各治疗组棘球蚴囊呈不同程度变性和坏死，与对照组比较差异有统计学意义（P〈0．01），40Gy与50Gy组，40Gy与60Gy组比较，病理改变差异有统计学意义（P〈0．01），但50Gy与60Gy组问比较病理改变差异无统计学意义（P〉0．05）；各照射组骨细胞均有不同程度变性、坏死现象。电镜下观察随照射剂量的升高，囊皮角质层及生发层结构遭到不同程度破坏。结论放射线治疗对骨包虫病的治疗有效，但需根据实际情况选择合适的照射剂量，预防不可逆性骨坏死的发生。
Objective Explore the efficacy that radiation （6WV-X-ray） treat bone hydatid disease. Methods Establish seventy gerbils bone hydatid disease animal models which were randomly divided into four groups, one group was to be control group, the animal models in other three groups were given 40 Gy/5 times, 50 Gy/5 times, 60 Gy/5 times, 6MV-X-ray positioning radiotherapy, 3 months later（the end of radiotherapy）, to observe the gerbils, general situation, to compare the mortality of scolex, protein and calcium concentration in capsule fluid, to observe the pathological changes of the horny layers and germinal layers, the varying of the bone cells in the irradiation areas and the ultrastructure of echinococcus granulosus cysts. Results Over the past 3 months, gerbils in each group has death cases, but the survival ones, general behaviors were normal. Among the groups, the difference of Che mortality of scolex（such as Control group： 22. 6, 40 Gy/5 times group： 96. 6, 50 Gy/5 times group： 131.5, 60 Gy/5 times group： 155.5 ） was statistically significant （P 〈 0. 01 ）. Protein concentration （calcimn ion concentration） in cyst fluid decrease （increase） with irradiation doses increase, The protein （calcium） concentrations are such as-the control group： 1. 0241 （2. 8875）, 40 Gy/5 time group： 0. 7245 （3. 0541）, 50 Gy/5 times group： 0. 6t71 （3. 5446） Gy to 60 Gy/5 times group： 0. 5058 （3. 8241 ）, which comgpared among each group there were significant differences （ P 〈 0. 01 ）. Histopathological results showed that echinococcus sac in the treatment groups showed different degrees of degeneration or necrosis, which compared with control group there were significant changing. Compared between 40 Gy and 50 Gy group, 40 Gy and 60 Gy group, the pathological changes of echinococcus sac were significant difference （ P 〈 0. 01 ）, but between 50 Gy and 60 Gy group the pathological changes is not obvious （P 〉 0. 05）. The bone ceils of the irradiated groups have been varying degrees of degeneration or necrosis. By electron microscopy, with the irradiation doses increase, the horny layers and germinal layers have been varying degrees of damage. Conclusion Radiation therapy is effective for bone hydatid disease, but need to select the appropriate radiation dose according to the actual situation, to prevent the occurrence of irreversible osteonecrosis.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第1期135-137,F0004,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（81060150）
新疆维吾尔自治区自然科学基金资助项目（2010211A43）
关键词
骨包虫病
模型
动物
放射治疗
Bone echinococcosis
Model, animal
Radiotherapy
