摘要
目的:研究当归补血汤对动脉血管内皮细胞凋亡的影响,探讨其可能作用机制。方法:当归补血汤提取液作用于血管紧张素Ⅱ(AngⅡ)诱导的大鼠动脉内皮细胞(RAOEC)凋亡模型(细胞密度1×105/mL)24 h,检测RAOEC凋亡率和半胱氨酸蛋白酶-8(caspase-8),Bcl-2相关x蛋白(Bax),B细胞淋巴瘤/白血病-2(Bcl-2)mRNA的表达。结果:浓度为500 mg.L-1的当归补血汤作用于AngⅡ诱导的RAOEC凋亡细胞后,凋亡率为(8.69±0.69)%,与对照组(14.45±1.87)%相比,凋亡率明显下降,且差异有统计学意义(P<0.05);当归补血汤组RAOECs的caspase-8,Bax mRNA水平,Bax mRNA/Bcl-2 mRNA分别为0.603±0.081,0.629±0.079,0.566±0.113,均明显低于对照组(1.083±0.115,1.024±0.072,1.018±0.111),且差异具有统计学意义(均为P<0.05)。结论:当归补血汤具有抗动脉血管内皮细胞凋亡的作用;当归补血汤可能通过下调caspase-8和Bax基因表达分别从死亡受体通路和线粒体通路抑制RAOEC凋亡。
Objective ： To study cells （RAOECs） and its mechanism. effect of Danggui Buxue decoction on apoptosis of rat arterial endothelial Method： Extracts of Danggui Buxue decoction was incubated with angiotensinll-induced apoptotic RAOECs （1 ×10^5/mL） for 24 hours, apoptosis rate and mRNA level of caspase-8, B cell lymphoma/leukmia-2 Bcl-2 and Bcl-2 assaciated x protein （Bax） were detected. Result： Apoptosis rate in 500 mgL-1 Danggui Buxue decoction group was （8.69 ±0.69）% , which significantly lower than that in control group （14.45 ± 1.87）% （P 〈0.05）; while mRNA level of caspase-8, Bax and Bcl-2 of Danggui Buxue decoction group （0. 603±0. 081, 0. 629 ±0. 079, 0. 566 ±0. 113, respectively） were all significantly lower than that in control group （1.083 ±0.115, 1.024 ±0.072, 1.018 ±0.111, respectively）, （all P 〈0.05）. Conclusion： Danggui Buxue decoction has effects on apoptosis of RAOECs. Danggui Buxue decoction may inhibit RAOECs apoptosis by down-regulating caspase-8 mRNA expression and mitochondrial signaling pathway by down- regulating Bax mRNA expression respectively.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第3期215-218,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江苏省中医药科技项目(LZ09099)
关键词
当归补血汤
动脉内皮细胞
凋亡
Danggui Buxue decoction
arterial endothelial cell
apoptosis
