摘要
目的:观察仙鹤草水提物在体外对血小板聚集、凝血试验及血液流变学的影响,探讨其对止血与凝血功能的作用及机制。方法:将0、4、20、80g/L仙鹤草水提物分别与全血、富血小板血浆(PRP)和贫血小板血浆(PPP)作用;分别以血小板聚集仪测定二磷酸腺苷(ADP)诱导的血小板聚集率,以流式细胞仪测定ADP诱导的血小板纤维蛋白原受体(Fg-R)和P-选择素表达水平;以血液凝固分析仪测定凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、纤维蛋白原(Fg),以硅化试管法测定凝血时间(CT),以电极法测定血浆Ca2+浓度,以乏因子血浆纠正试验测定凝血因子Ⅶ、Ⅷ、Ⅸ和Ⅺ活性;以血液流变仪测定全血黏度、血浆黏度及红细胞聚集指数。结果:4g/L组血小板聚集率、Fg-R和P-选择素表达率显著低于0g/L组(P<0.01)。血小板聚集率、Fg-R和P-选择素表达率与药物浓度呈显著负相关(P<0.01),三者抑制率与药物浓度呈显著正相关(P<0.01)。4g/L组APTT、CT和凝血因子FVII:C显著高于0g/L组(P均<0.01),PT、凝血因子FVIII:C、FIX:C和FXI:C分别显著低于0g/L组(P均<0.01)。APTT、CT、FⅦ:C与药物浓度呈显著正相关(P<0.01),PT、FVIII:C、FIX:C和FXI:C与药物浓度呈显著负相关(P<0.01)。4组间TT、Fg和Ca2+浓度无统计学差异(P>0.05)。4g/L组1s-1全血黏度、200s-1全血黏度、血浆黏度和红细胞聚集指数均显著高于0g/L组(P<0.01),四者水平均与浓度呈显著正相关(P<0.01)。结论:仙鹤草水提物可能通过抑制血小板Fg-R活化和释放反应途径而抑制血小板聚集以及可能通过抑制内源凝血途径而具有抗凝作用。仙鹤草水提物也可能通过活化外源凝血途径并增加血液黏度而具有促凝作用。
AIM： To observe the in vitro effects of Agrimoni Pilosa aqueous extract on platelet aggregation, coagulation function and hemorheology, and to explore its related mechanisms of action on hemostatic and coagulation function. METHODS： Platelet rich plasma （PRP） and platelet poor plasma （PPP） from volunteers were mixed with 0, 4 , 20 and 80 g/L Agrimoni Pilosa aqueous extract. The maximal platelet aggregation rate （PAR） of PRP induced by adenosine diphosphate （ADP） with platelet aggregation analyzer, expression levels of Pselection and fibrinogen receptor （Fg R） were detemined by Flow cytometry. Prothrombin time （PT）, activated partial thromboplastin time （APTT）, thrombin time （TT） and fibrinogen （Fg） of PPP were detected with blood coagulation analyzer, respectively, blood cloting time （CT） and plasma ionized calcium （Ca2＋） were detected with manual method and blood gas ana lyzer, respectively, coagulable factor VII, VIII, IX and XI were detected by poor factor plasma correcting test. Blood viscosity, plasma viscosity and index of red cell aggregation were meas ured with hemorheology analyzer. RESULTS：At 4 g/L group, PAR, FgR and Pselectin expression levels were significantly lower than those at 0 g/L group （R〈0.01）. There was negative correlation between extract dose and any one of PAR, FgR and Pselectin level, but positive correlation between extract dose and inhibitory rate of platelet aggregation （R〈0.01）. At 4 g/ L group, APTT, CT and FVII levels were remarkably higher , but PT, FVIII, FIX and FXI levels were lower, than those at 0 g/L group （R〈0.01）. APTT, CT and FVII levels showed positive correlation, but PT, FVIII, FIX and FXI levels showed negative correlation with extract dose （R〈0.01）. At4 g/Lgroup, levelsof blood viscosity （1 s1 and 200 sl）, plasma viscosity and index of red cell aggregation were markedly higher than those at 0 g/L group （R〈 0.01）. They all indicated positive correlation with extract dose （R 〈 0.01）. CONCLUSION： Agrirnoni Pilosa aqueous extract has anticoagu lation efficacy mainly via platelet aggregation and coagulation function inhibition caused by in hibition of platelet Fg-R activation, secretion reaction and blood coagulable factors of intrinsic pathway, respectively. The extract is able to promote blood coagulating via activating blood coagulable factors of extrinsic pathway and increasing blood viscosity.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2013年第1期10-16,共7页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
浙江省医学重点学科基金资助项目(07-010)
关键词
仙鹤草水提物
血小板聚集
凝血功能
血液流变学
受体
纤维蛋白原
凝血因子
Agrirnoni Pilosa
Platelet aggre- gation
Coagulation function
Hemorheology
Re- ceptor, Fibrinogen
Coagulable factors
