摘要
目的:观察P53基因在甲减大鼠肾脏中的表达变化,探讨凋亡在甲减肾损伤发病过程中的作用。方法:本研究在复制甲减大鼠模型的基础上,利用RT-PCR方法分析肾脏组织中P53mRNA的表达变化。结果:甲减组(HYPO)与对照组(CL)比较,血清TSH明显上升(P<0.05),TT3、TT4、FT3、FT4均较前明显下降(P<0.01),表示甲减模型复制成功。甲减大鼠肾脏组织中P53mRNA表达明显下降(P<0.05)。结论:低碘摄入可导致甲减,甲减会引起P53基因表达下降,进而导致肾脏细胞凋亡增加,引起肾脏损伤。
Objective：To observe the expression of P53 in hypothyroid rat kidney,study the effect of P53 on hypothyroid renal damage Methods：At the base of animal models of hypothyroidism,to observe the gene expression of P53 by using RT-PCR.Results：Compared with CL rat,TSH was significantly increased（P0.05）,TT3,TT4,FT4 and FT3 were significantly decreased（P0.01）,the levels of P53 were significantly decreased（P0.05） in the HYPO rats.Conclusion：Hypothyroid rat models can be produced successfully by being fed with low-iodine diet and drunk deionized water.Hypothyroidism can lead to lower gene expression of P53 in kidney.This finding suggests that apoptosis might induce hypothyroid renal damage.
出处
《中国医药导刊》
2012年第12期2169-2169,2171,共2页
Chinese Journal of Medicinal Guide
关键词
甲减肾脏
P53
Hypothyroidism kidney
P53 RT-PCR
