摘要
目的探讨雪旺细胞（SCs）对β-磷酸三钙／骨髓基质干细胞诱导分化的成骨细胞（β-TCP／BOOB）组织工程骨修复大鼠股骨缺损的影响。方法将SD大鼠骨髓基质干细胞（BMSCs）诱导分化为BDOB，并从SD乳鼠中提取SC8。将36只SD大鼠随机分为3组。每组12只，分别将术前预种植SC8的β-TCP／BOOB组织工程骨（A组）、β-TCP／BDOB组织工程骨（B组）、单纯β-TCP材料（C组）植入大鼠体内内修复长度为8mm的股骨缺损。于术后12周行x线摄片、组织学检查和显微CT扫描观察各组股骨缺部位化新生骨质的形成情况.结果术后12周x线片和显微CT扫描结果显示：A组大鼠骨缺．}!，!tZ完企愈合，B组1人鼠股骨缺拟部分愈合，c组大鼠股骨缺损术愈合。A组、B组、c组的x线影像。#坪分甲均分别为（12．08±0．90）、（9．25±1．06）、（6．17±1．12）分，3组比较差异有统计学意义（F=99．553、P=0．000），3组间两两比较差异均有统计学意义（P〈0．05）、组织学检查结果显示：A组植入物有大量骨质形成，广泛分布肥大的软骨细胞与成骨细胞；B组植入物中也可见散在新生骨形成，软骨细胞与成骨细胞散在稀疏分市；C组机人物中新生骨质形成极少，几乎未见软骨细胞或成骨细胞．结论SCs可提高B—TCP／BDOB组织工程骨修复大鼠股骨缺拟的效果。
Objective To explore the effect of Sehwann cells （SCs） on repair of femoral defects using a tissue engineeriug bone graft of β-tricalcium phosphate plus bone marrow stromal cell-derived osteoblast （β-TCP/BDOB） in a rat model. Methods Bone marrow stromal cells （BMSCs） collected from Sprague-Dawley （SD） rats were cultured and induced into BDOB in vitro. SCs were eolleeled from neonatal SI） rats. Thirty-six SI） rats were randomly divided into 3 even groups （ n = 12）. In group A. β-TCP/BDOB lisstle engineering grnfls loaded with SCs were implanted to repair the femoral defects of 8 mm. In groups B and C, tissue engineering bone grafts and β-TCP scaffolds were implanled without SCs into , respectively. X-ray detection, histological examination and Miero-CF detection were, weeks afler the implantation to evaluate new bone formation. Results X-ray and Miero-CT detections ated that tile femoral defects were completely bridged in group A, partly repaired in group B aM unhealed ill group C. The X-ray score was 32.08 ＋ O. 90 for group A, 9.25 ＋ 1.06 for group B and 6. 17 ± 1. 12 for group C, with a significant difference among the three （ F = 99. 553, P = 0. 000） and be- he-tween any 2 groups （ P 〈 O. 05） . The histological examinations showed abundant ossified tbrnmtion and a large number of hypertrophic osleoblasts and ehondroeytes in the implanted tissue engineering bone grafts in group A. scattered ossification sites and sparse osteoblasts and ehondrocytes in the implants in group B, few ossification sites and osteoblasts and chondrocytes in group C. Conclusion SCs ean improve the repairing effect of a β-TCP/BDOB tissue engineering bone graft on femoral defeets in rats.
出处
《中华创伤骨科杂志》
CAS
CSCD
北大核心
2013年第2期148-152,共5页
Chinese Journal of Orthopaedic Trauma
基金
国家重点尽础研究发展计划（973）资助项目（2009CB930003）
关键词
股骨
组织工程
雪旺细胞
缺损
大鼠
Femur Tissue engineering
Schwann cells
Defect： Rat
