摘要
旨在探讨枣树抗坏血酸过氧化物酶基因ZjAPX在植物渗透胁迫中的作用。将ZjAPX基因转入到模式植物拟南芥,以野生型(WT)、转ZjAPX拟南芥株系T2为试材,进行不同浓度NaCl胁迫和干旱胁迫。结果表明,转基因株系的种子萌发、植株生长均优于野生型株系;荧光定量PCR检测转基因拟南芥植株在干旱和盐胁迫处理10 d后目的基因ZjAPX的表达量显著高于野生拟南芥,表明ZjAPX的高表达明显提高了植株的抗旱和耐盐性。
In order to investigate the function of ZjAPX （ Zizyphus jujuba ascorbate peroxidase ） in response of plant to osmotic stress. In this study, ZjAPX gene was introduced into Arabidopsis. Wild type （ WT ） and transgenic type of Arabidopsis were used to the NaC1 osmotic stress and drought stress. These results suggested that transgenic seeds germination and seedlings growth were both better than the wild-type. At 10 days to be treated with NaC1 osmotic stress and drought stress, real-time quantitative PCR detection showed that the transcription level＂ of ZjAPX in transgenic plants was significantly higher than wide type. These results suggest that high expression of ZjAPX increased plant NaC1 osmotic stress and drought tolerance.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第1期78-82,共5页
Biotechnology Bulletin
基金
山西省自然科学基金项目(2010011038-2)
